Yu Lin-Zhong, Jiang Ai-Da, Cheng Yu-Yao, Lin Hui, Qin Qing-He, Ma Xiao-Dong
Nanfang Medical University, Guangzhou 510515, China.
Zhongguo Zhong Yao Za Zhi. 2006 Feb;31(3):220-3.
To study the effects of Liangge San to the expression of CD14 and scaverger receptor(SR) in the kupffer cells of liver and the pathological changes of liver tissue of endotoxemia-mice.
The model was established with intravenous injection of lipopolysaccharide (LPS) and at the same time different dose Liangge San were given. The expression of CD14 and scaverger receptor were detected with immunohigtochemistry at the 2nd, 4th, 8th hour ofter injury and analyzed with computer image system, and the pathological changes of liver tissue were also observed.
At the three different hours, the expression of CD14 and scaverger receptor in macrophages of liver of LPS-injury group showed significant increase and significant decrease respectively, compared with that of the blank-control group (P < 0.01). The expression in dexamethasone group and Liangge San different dose groups were intermediate between those in injury group and those in control group. Compared with expression of LPS-injury group, those of dexamethasone group and Liangge San different dose groups showed significant differences (P < 0.01), especially that of Liangge San high dose group. Liver cells showed vacuole change. Changes of CD14 and SR expression were paralleled with the severity of liver damages of the mice.
Liangge San can inhibite the up-regulation of CD14 expression and down-regulation of scaverger receptor expression in a dosage-dependent manner and also alleviate the damages of liver induced by LPS.
研究凉膈散对内毒素血症小鼠肝库普弗细胞CD14和清道夫受体（SR）表达及肝组织病理变化的影响。
采用静脉注射脂多糖（LPS）建立模型，同时给予不同剂量的凉膈散。于损伤后第2、4、8小时采用免疫组化法检测CD14和清道夫受体的表达，并用计算机图像系统进行分析，同时观察肝组织的病理变化。
在三个不同时间点，LPS损伤组肝巨噬细胞中CD14的表达显著增加，清道夫受体的表达显著降低，与空白对照组相比差异有统计学意义（P<0.01）。地塞米松组和凉膈散不同剂量组的表达介于损伤组和对照组之间。与LPS损伤组相比，地塞米松组和凉膈散不同剂量组的表达差异有统计学意义（P<0.01），尤以凉膈散高剂量组为著。肝细胞出现空泡样改变。CD14和SR表达的变化与小鼠肝损伤的严重程度平行。
凉膈散可剂量依赖性抑制CD14表达上调和清道夫受体表达下调，减轻LPS诱导的肝损伤。
