腺病毒介导的骨形态发生蛋白7转染对骨髓间充质干细胞成骨作用的影响
[Effect of adeno-BMP7 transfection on osteogenesis of BMSCs].
作者信息
Zhu Lian, Chen Fu-guo, Liu Wei, Cui Lei, Zhou Guang-dong, Liu De-li, Cao Yi-lin
机构信息
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiaotong Medical University and Shanghai Tissue Engineering Center, Shanghai 200011, China.
出版信息
Zhonghua Zheng Xing Wai Ke Za Zhi. 2006 Jan;22(1):59-62.
OBJECTIVE
To study the effect of adeno-BMP7 transfection on the biology of bone marrow stromal cells (BMSCs).
METHODS
Bone marrow was obtained from the goat. The BMSCs were isolated and cultured at the second passage. Once the cells attached and formed a monolayer with 70%-80% confluency, adeno-BMP7 (M.O.I. = 100) was added to the cells. After three days, calcium node was examined with staining; cell-coral compound was replanted subcutaneously.
RESULTS
With adeno-BMP7 transfection, BMP7 expression was detected with Western-blot; big calcium nodes were observed with staining. New bone formation was enhanced, which was evaluated by X-ray and histological examinations.
CONCLUSIONS
BMSCs transfected with adeno-BMP7 show much stronger osteogenic ability.
目的
研究腺病毒介导的骨形态发生蛋白7（adeno - BMP7）转染对骨髓基质细胞（BMSCs）生物学特性的影响。
方法
从山羊获取骨髓。分离培养BMSCs至第二代。待细胞贴壁并形成70% - 80%融合的单层后，向细胞中加入腺病毒介导的骨形态发生蛋白7（感染复数=100）。三天后，通过染色检测钙结节；将细胞 - 珊瑚复合物皮下再植。
结果
经腺病毒介导的骨形态发生蛋白7转染后，通过蛋白质免疫印迹法检测到骨形态发生蛋白7表达；染色观察到较大的钙结节。通过X射线和组织学检查评估，新骨形成增强。
结论
经腺病毒介导的骨形态发生蛋白7转染的骨髓基质细胞表现出更强的成骨能力。
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