Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, No. 639 Zhi Zhaoju Road, Shanghai 200011, P. R. China.
J Orthop Res. 2010 Mar;28(3):412-8. doi: 10.1002/jor.20973.
Segmental defect regeneration is still a clinical challenge. In this study, we investigated the feasibility of bone marrow stromal cells (BMSCs) infected with adenoviral vector containing the bone morphogenetic protein 7 gene (AdBMP7) and load-bearing to enhance bone regeneration in a critically sized femoral defect in the goat model. The defects were implanted with AdBMP7-infected BMSCs/coral (BMP7 group) or noninfected BMSCs/coral (control group), respectively, stabilized with an internal fixation rod and interlocking nails. Bridging of the segmental defects was evaluated by radiographs monthly, and confirmed by biomechanical tests. Much callus was found in the BMP7 group, and nails were taken off after 3 months of implantation, indicating that regenerated bone in the defect can be remodeled by load-bearing, whereas after 6 months in control group. After load-bearing, it is about 5 months; the mechanical property of newly formed bone in the BMP7 group was restored, but 8 months in control group. Our data suggested that the BMP7 gene-modified BMSCs and load-bearing can promote bone regeneration in segmental defects.
节段性缺损的再生仍然是一个临床挑战。在这项研究中，我们研究了感染含有骨形态发生蛋白 7 基因的腺病毒载体的骨髓基质细胞（BMSCs）和负载以增强山羊模型中临界尺寸股骨缺损骨再生的可行性。将 AdBMP7 感染的 BMSCs/珊瑚（BMP7 组）或未感染的 BMSCs/珊瑚（对照组）分别植入缺陷部位，并用内部固定杆和锁定钉固定。通过每月进行 X 线评估和生物力学测试来确认节段性缺损的桥接。在 BMP7 组中发现了大量骨痂，植入 3 个月后取出了钉子，表明缺损部位的再生骨可以通过负重进行重塑，而在对照组中则需要 6 个月。在负重后，大约需要 5 个月；BMP7 组中新形成的骨的机械性能得以恢复，但对照组则需要 8 个月。我们的数据表明，BMP7 基因修饰的 BMSCs 和负载可以促进节段性缺损中的骨再生。
