利用鸡的基因组序列进行火鸡（吐绶鸡）遗传标记的开发与定位。

Using the chicken genome sequence in the development and mapping of genetic markers in the turkey (Meleagris gallopavo).

作者信息

Chaves L D, Knutson T P, Krueth S B, Reed K M

机构信息

Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA.

出版信息

Anim Genet. 2006 Apr;37(2):130-8. doi: 10.1111/j.1365-2052.2005.01396.x.

DOI:10.1111/j.1365-2052.2005.01396.x

PMID:16573527

Abstract

The efficacy of employing the chicken genome sequence in developing genetic markers and in mapping the turkey genome was studied. Eighty previously uncharacterized microsatellite markers were identified for the turkey using BLAST alignment to the chicken genome. The chicken sequence was then used to develop primers for polymerase chain reaction where the turkey sequence was either unavailable or insufficient. A total of 78 primer sets were tested for amplification and polymorphism in the turkey, and informative markers were genetically mapped. Sixty-five (83%) amplified turkey genomic DNA, and 33 (42%) were polymorphic in the University of Minnesota/Nicholas Turkey Breeding Farms mapping families. All but one marker genetically mapped to the position predicted from the chicken genome sequence. These results demonstrate the usefulness of the chicken sequence for the development of genomic resources in other avian species.

摘要

研究了利用鸡基因组序列开发遗传标记以及绘制火鸡基因组图谱的功效。通过与鸡基因组进行BLAST比对，为火鸡鉴定出80个先前未表征的微卫星标记。然后，在火鸡序列不可用或不足的情况下，利用鸡序列开发用于聚合酶链反应的引物。总共测试了78组引物在火鸡中的扩增和多态性，并对信息性标记进行了遗传定位。在明尼苏达大学/尼古拉斯火鸡育种场的作图群体中，65个（83%）扩增了火鸡基因组DNA，33个（42%）具有多态性。除一个标记外，所有标记均在遗传上定位到了根据鸡基因组序列预测的位置。这些结果证明了鸡序列在开发其他禽类基因组资源方面的有用性。