大肠杆菌蔗糖通透酶中参与糖/H(+) 同向转运的残基相对于乳糖通透酶的保守性。
Conservation of residues involved in sugar/H(+) symport by the sucrose permease of Escherichia coli relative to lactose permease.
作者信息
Vadyvaloo Viveka, Smirnova Irina N, Kasho Vladimir N, Kaback H Ronald
机构信息
Department of Physiology and Microbiology, Immunology and Molecular Genetics, Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095-7327, USA.
出版信息
J Mol Biol. 2006 May 12;358(4):1051-9. doi: 10.1016/j.jmb.2006.02.050. Epub 2006 Mar 9.
Abstract
Building a three-dimensional model of the sucrose permease of Escherichia coli (CscB) with the X-ray crystal structure lactose permease (LacY) as template reveals a similar overall fold for CscB. Moreover, despite only 28% sequence identity and a marked difference in substrate specificity, the structural organization of the residues involved in sugar-binding and H(+) translocation is conserved in CscB. Functional analyses of mutants in the homologous key residues provide strong evidence that they play a similar critical role in the mechanisms of CscB and LacY.
摘要
以X射线晶体结构乳糖通透酶（LacY）为模板构建大肠杆菌蔗糖通透酶（CscB）的三维模型，结果显示CscB具有相似的整体折叠结构。此外，尽管CscB与LacY的序列同一性仅为28%，且底物特异性存在显著差异，但参与糖结合和H(+)转运的残基的结构组织在CscB中是保守的。对同源关键残基突变体的功能分析提供了有力证据，表明它们在CscB和LacY的机制中发挥相似的关键作用。
相似文献
1
Conservation of residues involved in sugar/H(+) symport by the sucrose permease of Escherichia coli relative to lactose permease.大肠杆菌蔗糖通透酶中参与糖/H(+) 同向转运的残基相对于乳糖通透酶的保守性。
J Mol Biol. 2006 May 12;358(4):1051-9. doi: 10.1016/j.jmb.2006.02.050. Epub 2006 Mar 9.
2
Sugar recognition by CscB and LacY.CscB 和 LacY 对糖的识别。
Biochemistry. 2011 Dec 27;50(51):11009-14. doi: 10.1021/bi201592y. Epub 2011 Dec 1.
3
Sequence alignment and homology threading reveals prokaryotic and eukaryotic proteins similar to lactose permease.序列比对和同源穿线法揭示了与乳糖通透酶相似的原核生物和真核生物蛋白质。
J Mol Biol. 2006 May 12;358(4):1060-70. doi: 10.1016/j.jmb.2006.02.049. Epub 2006 Mar 9.
4
Lactose permease and the alternating access mechanism.乳糖渗透酶和交替访问机制。
Biochemistry. 2011 Nov 15;50(45):9684-93. doi: 10.1021/bi2014294. Epub 2011 Oct 19.
5
Probing the periplasmic-open state of lactose permease in response to sugar binding and proton translocation.探测乳糖透过酶在结合糖和质子转移时的周质开放状态。
J Mol Biol. 2010 Dec 3;404(3):506-21. doi: 10.1016/j.jmb.2010.09.045. Epub 2010 Sep 25.
6
Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition.乳糖通透酶与亲和失活剂复合物的晶体结构为糖识别提供了独特的见解。
Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9361-6. doi: 10.1073/pnas.1105687108. Epub 2011 May 18.
7
Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane.糖结合在纯化的乳糖通透酶（LacY）中诱导的全局构象变化与在天然细菌膜中相同。
Proc Natl Acad Sci U S A. 2010 May 25;107(21):9903-8. doi: 10.1073/pnas.1004515107. Epub 2010 May 10.
8
Monoclonal antibody 4B1 influences the pK of Glu325 in lactose permease (LacY) from Escherichia coli: evidence from SEIRAS.单克隆抗体 4B1 影响大肠杆菌乳糖通透酶（LacY）中 Glu325 的 pK：来自 SEIRAS 的证据。
FEBS Lett. 2020 Oct;594(20):3356-3362. doi: 10.1002/1873-3468.13907. Epub 2020 Aug 30.
9
It takes two to tango: The dance of the permease.二人转：通透酶的舞蹈。
J Gen Physiol. 2019 Jul 1;151(7):878-886. doi: 10.1085/jgp.201912377. Epub 2019 May 30.
10
Binding affinity of lactose permease is not altered by the H+ electrochemical gradient.乳糖通透酶的结合亲和力不会因H⁺电化学梯度而改变。
Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12148-52. doi: 10.1073/pnas.0404936101. Epub 2004 Aug 10.
引用本文的文献
1
Cyanobacteria as cell factories for the photosynthetic production of sucrose.蓝细菌作为用于光合生产蔗糖的细胞工厂。
Front Microbiol. 2023 Feb 14;14:1126032. doi: 10.3389/fmicb.2023.1126032. eCollection 2023.
2
Enzymatic and structural characterization of β-fructofuranosidase from the honeybee gut bacterium Frischella perrara.β-呋喃果糖苷酶的酶学和结构特征研究，该酶来自于蜜蜂肠道细菌弗氏乳杆菌。
Appl Microbiol Biotechnol. 2022 Apr;106(7):2455-2470. doi: 10.1007/s00253-022-11863-9. Epub 2022 Mar 10.
3
Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO.基于快速生长的蓝藻UTEX 2973构建并分析人工菌群，以从CO生产平台化学品3-羟基丙酸。
Biotechnol Biofuels. 2020 May 6;13:82. doi: 10.1186/s13068-020-01720-0. eCollection 2020.
4
Structural and Functional Adaptability of Sucrose and Lactose Permeases from to the Membrane Lipid Composition.到膜脂组成的蔗糖和乳糖通透酶的结构和功能适应性。
Biochemistry. 2020 May 19;59(19):1854-1868. doi: 10.1021/acs.biochem.0c00174. Epub 2020 May 7.
5
Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973.在快速生长的蓝藻集胞藻 UTEX 2973 中增强蔗糖的生产。
Sci Rep. 2020 Jan 15;10(1):390. doi: 10.1038/s41598-019-57319-5.
6
Structure of LacY with an α-substituted galactoside: Connecting the binding site to the protonation site.带有α-取代半乳糖苷的LacY结构：连接结合位点与质子化位点。
Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):9004-9. doi: 10.1073/pnas.1509854112. Epub 2015 Jul 8.
7
Modelling and mutational analysis of Aspergillus nidulans UreA, a member of the subfamily of urea/H⁺ transporters in fungi and plants.构巢曲霉UreA的建模与突变分析，UreA是真菌和植物中尿素/H⁺转运蛋白亚家族的成员。
Open Biol. 2014 Jun;4(6):140070. doi: 10.1098/rsob.140070.
8
Galactoside-binding site in LacY.乳糖苷结合位点在 LacY 中。
Biochemistry. 2014 Mar 11;53(9):1536-43. doi: 10.1021/bi401716z. Epub 2014 Feb 26.
9
Use of molecular modelling to probe the mechanism of the nucleoside transporter NupG.利用分子建模探究核苷转运蛋白NupG的机制。
Mol Membr Biol. 2013 Mar;30(2):114-28. doi: 10.3109/09687688.2012.748939. Epub 2012 Dec 21.
10
Proton-coupled dynamics in lactose permease.乳糖通透酶中的质子偶联动力学。
Structure. 2012 Nov 7;20(11):1893-904. doi: 10.1016/j.str.2012.08.021. Epub 2012 Sep 20.
本文引用的文献
1
Sequence alignment and homology threading reveals prokaryotic and eukaryotic proteins similar to lactose permease.序列比对和同源穿线法揭示了与乳糖通透酶相似的原核生物和真核生物蛋白质。
J Mol Biol. 2006 May 12;358(4):1060-70. doi: 10.1016/j.jmb.2006.02.049. Epub 2006 Mar 9.
2
TCDB: the Transporter Classification Database for membrane transport protein analyses and information.TCDB：用于膜转运蛋白分析和信息查询的转运蛋白分类数据库。
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D181-6. doi: 10.1093/nar/gkj001.
3
Interhelical packing modulates conformational flexibility in the lactose permease of Escherichia coli.螺旋间堆积调节大肠杆菌乳糖通透酶的构象灵活性。
Biochemistry. 2005 May 31;44(21):7669-77. doi: 10.1021/bi0502801.
4
Exploiting luminescence spectroscopy to elucidate the interaction between sugar and a tryptophan residue in the lactose permease of Escherichia coli.利用发光光谱法阐明糖与大肠杆菌乳糖通透酶中色氨酸残基之间的相互作用。
Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12706-11. doi: 10.1073/pnas.1835645100. Epub 2003 Oct 17.
5
Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli.大肠杆菌3-磷酸甘油转运蛋白的结构与机制
Science. 2003 Aug 1;301(5633):616-20. doi: 10.1126/science.1087619.
6
Structure and mechanism of the lactose permease of Escherichia coli.大肠杆菌乳糖通透酶的结构与机制
Science. 2003 Aug 1;301(5633):610-5. doi: 10.1126/science.1088196.
7
A mutation in the lactose permease of Escherichia coli that decreases conformational flexibility and increases protein stability.大肠杆菌乳糖通透酶中的一种突变，该突变降低了构象灵活性并增加了蛋白质稳定性。
Biochemistry. 2003 Mar 18;42(10):3025-31. doi: 10.1021/bi027329c.
8
Aromatic stacking in the sugar binding site of the lactose permease.乳糖通透酶糖结合位点中的芳香堆积作用
Biochemistry. 2003 Feb 18;42(6):1377-82. doi: 10.1021/bi027152m.
9
Manipulating conformational equilibria in the lactose permease of Escherichia coli.调控大肠杆菌乳糖通透酶中的构象平衡。
J Mol Biol. 2002 Jan 25;315(4):561-71. doi: 10.1006/jmbi.2001.5289.
10
The C-4 hydroxyl group of galactopyranosides is the major determinant for ligand recognition by the lactose permease of Escherichia coli.吡喃半乳糖苷的C-4羟基是大肠杆菌乳糖通透酶识别配体的主要决定因素。
Biochemistry. 2001 Oct 30;40(43):13015-9. doi: 10.1021/bi011233l.
Zotero 插件