L-氨基酸在高效液相色谱法测定石杉碱甲中的应用。

Application of L-Amino acid in determination of Huperzine A by high performance liquid chromatography.

作者信息

Pan Jian, Jin Risheng, Hu Xueqiao

机构信息

Key Laboratory of Bio-process, Hefei University of Technology, Ministry of Education, Hefei, Anhui 230009, PR China.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2006 May 19;836(1-2):108-10. doi: 10.1016/j.jchromb.2006.03.018. Epub 2006 Mar 29.

DOI:10.1016/j.jchromb.2006.03.018

PMID:16574511

Abstract

A selective HPLC method for determination of Huperzine A in Huperzia serrata Extract has been developed and validated. Huperzine A was dissolved in 0.01 mol/L HCl, chromatographed on an Agilent Zorbax SB-C18 (150 mm x 4.6 mm i.d., 5 microm) column, with a mobile phase consisting of methanol-1mM L-Lysine water solution (50:50, v/v), and detected at 310 nm. The UV peak areas were used for quantitation of Huperzine A content. The correlation coefficient (R(2)) of the calibration was 0.9999 over the range of 1-25 microg/ml and intra- and interday precision over this range were not more than 2%. The method was successfully applied to characterize and determine the Huperzine A in Huperzia serrata Extract.

摘要

已开发并验证了一种用于测定蛇足石杉提取物中石杉碱甲的选择性高效液相色谱法。石杉碱甲溶解于0.01mol/L盐酸中，在安捷伦Zorbax SB-C18（150mm×4.6mm内径，5微米）柱上进行色谱分析，流动相由甲醇-1mM L-赖氨酸水溶液（50:50，v/v）组成，并在310nm处检测。紫外峰面积用于定量石杉碱甲含量。校准曲线在1-25μg/ml范围内的相关系数（R²）为0.9999，此范围内的日内和日间精密度均不超过2%。该方法成功应用于蛇足石杉提取物中石杉碱甲的表征和测定。