Ba Boubakar B, Nso Branly Bikie Bi, Quentin Claudine, Saux Marie-Claude
Pharmacokinetic and Clinical Pharmacy Laboratory, (EA 525), Victor Segalen Bordeaux 2 University, Bordeaux, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jul 1;854(1-2):104-8. doi: 10.1016/j.jchromb.2007.04.011. Epub 2007 Apr 19.
An isocratic high-performance liquid chromatography (HPLC) method with on-line extraction has been developed to determine linezolid in Mueller-Hinton broth. The loading mobile phase consisting of water-acetonitrile 99:1 (v/v) allowed retention of the analyte on a LiChrocart 4-4 pre-column filled with a LiChrospher 100 RP-8, 5 microm. The transfer of the analyte by a backflush mode to a 150 mm x 4.6 mm I.D. Kromasil C8 5 microm column was performed using a mobile phase of water-acetonitrile 80:20 (v/v). UV detection at 254 nm allowed a quantification limit of 0.39 microg/mL with a 50-microL sample size. The method was successfully applied to in vitro pharmacokinetic-pharmacodynamic studies.
已开发出一种具有在线萃取功能的等度高效液相色谱（HPLC）方法，用于测定穆勒-欣顿肉汤中的利奈唑胺。由水-乙腈99:1（v/v）组成的进样流动相可使分析物保留在填充有5微米LiChrospher 100 RP-8的LiChrocart 4-4预柱上。使用水-乙腈80:20（v/v）的流动相，通过反冲模式将分析物转移至150 mm×4.6 mm内径的5微米Kromasil C8柱上。在254 nm处进行紫外检测，50微升进样量时的定量限为0.39微克/毫升。该方法已成功应用于体外药代动力学-药效学研究。
