The electrochemical proton potential of Bacillus alcalophilus.

Bacillus alcalophilus strain ATCC 27647 showed usual growth characteristics, when inoculated at pH 10.4. The cells entered the logarithmic phase at pH 10.3, and as growth continued, the pH dropped further to a value of 8.8 in the stationary phase. B. alcalophilus strain DSM 485 showed comparable growth only in the initial phase after the addition to fresh medium. The small initial growth period was succeeded by a long lag phase, where the pH continuously dropped. The cells resumed growth after the pH was about 10.0 and continued to grow accompanied by a further decrease of external pH. The bioenergetic parameters measured in the initial growth phase of the two strains at high pH (10.1-10.3) were nearly the same, i.e. delta pH = +97 to +110 mV, delta psi = -206 to -213 mV and delta microH+ = -109 to -103 mV. The inverted proton gradient of about 1.7-1.9 at high pH decreased, as the external pH dropped during growth. This led to an increase of the proton motive force (delta microH+), although the membrane potential (delta psi) also declined. The ATP/ADP ratio of strain DSM 485 was high (4.5-5.5) at fast growth during the initial and second growth period. The ratio declined to about 1.5 at the end of the lag phase. At the initial growth phase and at the end of the lag phase, the delta microH+ was, however, the same (approximately -106 mV) and considerably lower than in the middle of the second growth period (approximately -140 mV). Fast growth, therefore, correlates with a high ATP/ADP ratio but not necessarily with a high delta microH+. Addition of gramicidin or carbonylcyanide m-chlorophenylhydrazone stopped growth of B. alcalophilus strain DSM 485 at pH 10.3 or 9.5 and gramicidin immediately decreased the internal ATP/ADP ratio from 4.5 to 1.2 at pH 10.3.