McKibbin P E, Cotton S J, McMillan S, Holloway B, Mayers R, McCarthy H D, Williams G
Department of Medicine, University of Liverpool, UK.
Diabetes. 1991 Nov;40(11):1423-9. doi: 10.2337/diab.40.11.1423.
Neuropeptide Y (NPY) concentrations were measured by radioimmunoassay in eight microdissected hypothalamic regions of obese (fa/fa) and lean (Fa/?) Zucker rats. Freely fed obese rats showed significant (40-100%) increases in NPY concentrations in several regions, notably the paraventricular, ventromedial, and dorsomedial nuclei and the arcuate nucleus/median eminence, compared with lean rats. Hypothalamic NPY concentrations were not affected in either obese or lean rats by food restriction, which caused 25% weight loss over 3 wk. Refeeding to initial weight significantly increased NPY levels in the ventromedial and dorsomedial nuclei in lean rats but did not significantly alter NPY concentrations in any hypothalamic region in obese rats. These observations indicate fundamental differences in the regulation of hypothalamic NPY between obese and lean Zucker rats. NPY injected into the paraventricular nucleus and other regions causes hyperphagia, obesity, and increased secretion of insulin, glucagon, ACTH, and corticosterone. These behavioral and neuroendocrine abnormalities all occur in the obese Zucker syndrome and may be due to increased NPY-ergic activity in the hypothalamus.
采用放射免疫分析法测定了肥胖型（fa/fa）和瘦型（Fa/?） Zucker大鼠下丘脑八个显微切割区域的神经肽Y（NPY）浓度。自由进食的肥胖大鼠与瘦大鼠相比，几个区域的NPY浓度显著升高（40%-100%），尤其是室旁核、腹内侧核、背内侧核以及弓状核/正中隆起。食物限制（3周内体重减轻25%）对肥胖大鼠和瘦大鼠的下丘脑NPY浓度均无影响。重新喂食至初始体重后，瘦大鼠腹内侧核和背内侧核的NPY水平显著升高，但肥胖大鼠下丘脑任何区域的NPY浓度均无显著变化。这些观察结果表明肥胖型和瘦型Zucker大鼠下丘脑NPY调节存在根本差异。向室旁核及其他区域注射NPY会导致食欲亢进、肥胖以及胰岛素、胰高血糖素、促肾上腺皮质激素和皮质酮分泌增加。这些行为和神经内分泌异常均出现在肥胖型Zucker综合征中，可能是由于下丘脑NPY能活性增加所致。
