阿尔茨海默病血沉棕黄层接种所致仓鼠脑内细胞质神经丝聚集的特征：与铝中毒引起的实验性神经原纤维变化的比较

Characterization of intracytoplasmic neurofilament accumulation in hamster brain caused by Alzheimer buffy coat inoculation: comparison with experimental neurofibrillary changes produced by aluminum intoxication.

作者信息

Takeda M, Nishimura T, Kudo T, Tanimukai S, Gotow T, Tanabe H, Miki T, Nakamura Y, Niigawa H, Morita H

机构信息

Department of Neuropsychiatry, Osaka University Medical School, Japan.

出版信息

Gerontology. 1991;37 Suppl 1:31-42. doi: 10.1159/000213295.

DOI:10.1159/000213295

PMID:1657730

Abstract

Neurofilament accumulation was produced in neuronal perikarya of the lower brain stem nuclei in the hamster brain by inoculating buffy coat from a patient with Alzheimer's disease. The abnormally proliferated neurofilament was characterized by immunostaining and electron microscopy. The intracytoplasmic neurofilament accumulation was positively stained with anti-tau and anti-ubiquitin, but not with antibody against paired helical filaments. Experimental neurofibrillary changes were produced in the rabbit brain by aluminum injection, and the proliferated neurofilaments in the rabbit brain were compared with those in the hamster brain inoculated with Alzheimer buffy coat. The results indicate that the long pathological process in the hamster brain is triggered by Alzheimer's buffy coat inoculation which results in intracytoplasmic accumulation of modified neurofilaments.

摘要

通过接种阿尔茨海默病患者的血沉棕黄层，在仓鼠脑桥脑下部神经细胞核周产生神经丝积聚。通过免疫染色和电子显微镜对异常增殖的神经丝进行表征。胞浆内神经丝积聚用抗tau和抗泛素抗体呈阳性染色，但用抗双螺旋丝抗体染色则为阴性。通过注射铝在兔脑中产生实验性神经原纤维变化，并将兔脑中增殖的神经丝与接种阿尔茨海默血沉棕黄层的仓鼠脑中的神经丝进行比较。结果表明，仓鼠脑中漫长的病理过程是由接种阿尔茨海默血沉棕黄层引发的，这导致了修饰神经丝在胞浆内的积聚。