Van Rensburg C E, Van Staden A M, Anderson R
Department of Immunology, Faculty of Medicine, University of Pretoria, Republic of South Africa.
Free Radic Biol Med. 1991;11(3):285-91. doi: 10.1016/0891-5849(91)90125-m.
The effects of the phagocyte-derived reactive oxidants hydrogen peroxide (H2O2) and hypochlorous acid (HOC1) on the activity of poly(ADP-ribose) polymerase (pADP RP), an enzyme involved in DNA repair, and on the induction and repair of DNA strand breaks in human mononuclear leukocytes (MNL) have been investigated in vitro. Exposure of MNL to reagent H2O2 was accompanied by DNA damage and activation of pADP RP. Addition of reagent HOCl (25 microM) was not associated with DNA strand breaks. However, when combined with 150 microM H2O2, HOCl potentiated H2O2-mediated DNA damage, and compromised the repair process. Furthermore, HOCl caused a dose-related decrease in the activity of pADP RP in both control and H2O2-exposed MNL. Interactions between the phagocyte-derived reactive oxidants H2O2 and HOCl are probably involved in the etiology of inflammation-related cancer.
体外研究了吞噬细胞衍生的活性氧化剂过氧化氢（H2O2）和次氯酸（HOC1）对聚（ADP - 核糖）聚合酶（pADP RP）活性的影响，pADP RP是一种参与DNA修复的酶，还研究了其对人单核白细胞（MNL）中DNA链断裂的诱导和修复的影响。将MNL暴露于试剂H2O2会伴随着DNA损伤和pADP RP的激活。添加试剂HOCl（25 microM）与DNA链断裂无关。然而，当与150 microM H2O2联合使用时，HOCl增强了H2O2介导的DNA损伤，并损害了修复过程。此外，HOCl导致对照和H2O2暴露的MNL中pADP RP的活性呈剂量相关下降。吞噬细胞衍生的活性氧化剂H2O2和HOCl之间的相互作用可能与炎症相关癌症的病因有关。
