都柏林沙门氏菌lane质粒pSDL2的EcoRI缺失衍生物9-18 delta 7的Tn1725转座子诱变
Tn1725 transposon mutagenesis of 9-18 delta 7, an EcoRI deletion derivative of Salmonella dublin lane plasmid pSDL2.
作者信息
Daifuku R, Chikami G K
机构信息
Department of Medicine, University of California, Los Angeles 90024-1688.
出版信息
Infect Immun. 1991 Dec;59(12):4720-3. doi: 10.1128/iai.59.12.4720-4723.1991.
Abstract
A 37.5-kb derivative of the Salmonella dublin virulence plasmid pSDL2 was subjected to mutagenesis with the transposon Tn1725. Fifty-two insertions were mapped, and the mutants were tested for their ability to restore virulence to a plasmid-free strain of S. dublin. Twenty-nine of these inserts could not restore full virulence and thus define nine regions of the plasmid essential for virulence. Deletion of a 4.5-kb region by Bal31 nuclease resulted in a 33-kb derivative that maintained full virulence.
摘要
用转座子Tn1725对鼠伤寒沙门氏菌都柏林毒力质粒pSDL2的一个37.5kb衍生物进行诱变。绘制了52个插入位点的图谱，并测试了这些突变体恢复无质粒鼠伤寒沙门氏菌都柏林菌株毒力的能力。其中29个插入不能恢复完全毒力，因此确定了质粒上对毒力至关重要的9个区域。用Bal31核酸酶缺失一个4.5kb区域产生了一个保持完全毒力的33kb衍生物。
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