Finlay B B, Heffron F, Falkow S
Department of Medical Microbiology, Stanford University School of Medicine, CA 94305.
Science. 1989 Feb 17;243(4893):940-3. doi: 10.1126/science.2919285.
Salmonella bacteria are capable of entering (invading) and multiplying within eukaryotic cells. Stable adherence to and invasion of epithelial cells by S. choleraesuis and S. typhimurium were found to require de novo synthesis of several new bacterial proteins. This inducible event appears to be a coordinately regulated system dependent on trypsin- and neuraminidase-sensitive structures present on the epithelial cell surface. Mutants of S. choleraesuis and S. typhimurium were unable to synthesize these proteins and did not stably adhere to nor invade eukaryotic cells. Two such S. typhimurium mutants were avirulent in mice, an indication that these proteins are required for Salmonella virulence.
沙门氏菌能够进入（侵袭）真核细胞并在其中繁殖。研究发现，猪霍乱沙门氏菌和鼠伤寒沙门氏菌对上皮细胞的稳定黏附及侵袭需要从头合成几种新的细菌蛋白。这种可诱导事件似乎是一个协调调控的系统，依赖于上皮细胞表面存在的对胰蛋白酶和神经氨酸酶敏感的结构。猪霍乱沙门氏菌和鼠伤寒沙门氏菌的突变体无法合成这些蛋白，也不能稳定黏附或侵袭真核细胞。两个这样的鼠伤寒沙门氏菌突变体在小鼠中无致病性，这表明这些蛋白是沙门氏菌致病所必需的。
