促前胸腺激素对昆虫前胸腺的体外激活作用。
In vitro activation of insect prothoracic glands by the prothoracicotropic hormone.
作者信息
Bollenbacher W E, Agui N, Granger N A, Gilbert L I
机构信息
Department of Biological Sciences, Northwestern University, Evanston, Illinois 60201.
出版信息
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5148-52. doi: 10.1073/pnas.76.10.5148.
An in vitro assay for the prothoracicotropic hormone has been developed that utilizes an ecdysone radioimmunoassay to quantify the increase in the rate of ecdysone synthesis elicited by the neurohormonal activation of the prothoracic glands. The rapidity, reproducibility, and accuracy of the assay were maximized by using one member of a gland pair as the control and the other as the test gland. This was possible because the basal rates of ecdysone synthesis by the members of a gland pair were equivalent. Activation was demonstrated to be dose dependent and specific, with prothoracicotropic hormone activity present only in homogenates of brain. The in vitro activation of the prothoracic glands was verified with the Manduca bioassay for the prothoracicotropic hormone in which the morphological responses to the hormone were correlated with increased in vivo ecdysone titers. These results provide unequivocal evidence that the activation of the prothoracic glands by the prothoracicotropic hormone is direct and suggest that activation represents an increase in a basal rate of ecdysone synthesis.
已开发出一种促前胸腺激素的体外测定法，该方法利用蜕皮激素放射免疫测定法来量化前胸腺神经激素激活引发的蜕皮激素合成速率的增加。通过使用腺体对中的一个成员作为对照，另一个作为测试腺体，使该测定法的速度、可重复性和准确性最大化。这是可行的，因为腺体对成员的蜕皮激素合成基础速率是相等的。已证明激活是剂量依赖性和特异性的，促前胸腺激素活性仅存在于脑匀浆中。前胸腺的体外激活通过用于促前胸腺激素的烟草天蛾生物测定法得到验证，在该测定法中，对该激素的形态学反应与体内蜕皮激素滴度的增加相关。这些结果提供了明确的证据，表明促前胸腺激素对前胸腺的激活是直接的，并表明激活代表蜕皮激素合成基础速率的增加。
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