Vedeckis W V, Bollenbacher W E, Gilbert L I
Mol Cell Endocrinol. 1976 Jun-Jul;5(1-2):81-8. doi: 10.1016/0303-7207(76)90072-1.
The prothoracic glands of the tobacco hornworm, Manduca sexta, were studied to determine if cyclic AMP is involved in the regulation of alpha-ecdysone secretion. Culturing glands in the presence of the phosphodiesterase inhibitors, aminophylline and 1-methyl-3-isobutylxanthine, caused a greater than 2-fold stimulation of ecdysone secretion while cyclic AMP alone was ineffective. Based on a dose-response analysis, 1-methyl-3-isobutylxanthine was 200 times more potent than aminophylline. Measurements of endogenous prothoracic gland cyclic AMP during the fifth larval instar demonstrated that dramatically increased levels preceded the increase in in vitro ecdysone-secretory ability. The data suggest that cyclic AMP may act as a second messenger in the stimulation of prothoracic gland alpha-ecdysone secretion by the prothoracicotropic brain hormone.
为了确定环磷酸腺苷（cAMP）是否参与α-蜕皮激素分泌的调节，研究了烟草天蛾（烟草天蛾）的前胸腺。在磷酸二酯酶抑制剂氨茶碱和1-甲基-3-异丁基黄嘌呤存在的情况下培养腺体，可使蜕皮激素分泌受到2倍以上的刺激，而单独使用环磷酸腺苷则无效。基于剂量反应分析，1-甲基-3-异丁基黄嘌呤的效力比氨茶碱高200倍。在第五龄幼虫期间对前胸腺内源性环磷酸腺苷的测量表明，在体外蜕皮激素分泌能力增加之前，其水平显著升高。数据表明，环磷酸腺苷可能作为一种第二信使，在前胸腺促激素刺激前胸腺α-蜕皮激素分泌过程中发挥作用。
