Li Ying-li, Bai Fang, Shan Zhi-ying, Xu Hai-jin, Qiao Ming-qiang
Institute for Molecular Biology, Nankai University, Tianjin 300071, China.
Wei Sheng Wu Xue Bao. 2006 Feb;46(1):18-22.
Pseudomonas aeruginosa, a typical opportunistic pathogen, has a single polar flagella which provides swimming motility and virulence. Twelve of insertional mutants with Mu transposition complexes into genomic DNA of Pseudomonas aeruginosa were screened on the changes of swimming motility. After gene cloning and DNA sequencing of those mutants, ten of them were illustrated that defective mutations on swimming motility were caused by insertions of Mu transposon into 10 of different functional genes which may involved in biosynthesis of flagella and gene regulations, but another two mutants were resulted from the insertion of two new genes (PA2950, PA5022) with function unknown. Observation of these two mutants with an integrity flagellum under electron microscope showed that two new genes were not involved in construction of flagella, but probably in energy metabolism and gene regulation of swimming motility.
铜绿假单胞菌是一种典型的机会致病菌，具有一根极鞭毛，该鞭毛赋予其游动能力和毒力。根据游动能力的变化，筛选出了12个携带Mu转座复合物插入铜绿假单胞菌基因组DNA的插入突变体。对这些突变体进行基因克隆和DNA测序后发现，其中10个突变体游动能力的缺陷突变是由Mu转座子插入10个不同的功能基因所致，这些基因可能参与鞭毛的生物合成和基因调控，但另外两个突变体是由两个功能未知的新基因（PA2950、PA5022）插入导致的。在电子显微镜下观察这两个具有完整鞭毛的突变体发现，这两个新基因不参与鞭毛的构建，但可能参与游动能力的能量代谢和基因调控。
