Hwang S T, Wachter C, Schatz G
Biocenter, University of Basel, Switzerland.
J Biol Chem. 1991 Nov 5;266(31):21083-9.
Import of authentic or artificial precursor proteins into the matrix of isolated yeast mitochondria can proceed via a translocation intermediate that is lodged between the two mitochondrial membranes. The intermediate accumulates when import is arrested by depleting mitochondria of ATP. Generation of the intermediate requires a potential across the inner membrane. The intermediate is membrane-bound, partly or completely processed (depending on the precursor), and chased into the matrix by added ATP. This chase does not require a potential across the inner membrane. The properties of this intermediate support the proposal (Hwang, S., Jascur, J., Vestweber, D., Pon, L., and Schatz, G. (1989) J. Cell Biol. 109, 487-493) that import into the matrix involves two distinct translocation systems in the outer and the inner mitochondrial membrane that are not permanently coupled to each other. Only translocation across the inner membrane requires ATP in the matrix.
将真实的或人工合成的前体蛋白导入分离的酵母线粒体基质中可通过一种位于两个线粒体膜之间的易位中间体进行。当通过耗尽线粒体中的ATP来阻止导入时，该中间体会积累。中间体的产生需要内膜两侧的电位。该中间体与膜结合，部分或完全被加工（取决于前体），并通过添加ATP被追踪到基质中。这种追踪不需要内膜两侧的电位。该中间体的特性支持了以下提议（Hwang, S., Jascur, J., Vestweber, D., Pon, L., and Schatz, G. (1989) J. Cell Biol. 109, 487 - 493），即导入基质涉及线粒体外膜和内膜中两个不同的易位系统，它们并非永久相互偶联。只有跨内膜的易位需要基质中的ATP。
