Quadrini Karen J, Bieker James J
The Brookdale Department of Molecular, Cell and Developmental Biology, Mount Sinai School of Medicine, New York, NY 10029, USA.
FEBS Lett. 2006 Apr 17;580(9):2285-93. doi: 10.1016/j.febslet.2006.03.039. Epub 2006 Mar 24.
Erythroid Krüppel-like factor (EKLF/KLF1) is an erythroid specific, C(2)H(2) zinc finger transcription factor that is essential for the proper chromatin structure and expression of the adult beta-globin gene. Herein, we determine that 26S proteasome inhibitors lead to an accumulation of EKLF protein in murine erythroleukemia (MEL) cells. In addition, EKLF half-life in both MEL cells (<3h) and fetal liver cells (between 6 and 9h) is stabilized in the presence of these inhibitors. EKLF is ubiquitinated in vivo, however its modification does not rely on a particular internal lysine. Finally, EKLF contains two PEST sequences within its N-terminus that have no effect on the ability of EKLF to be ubiquitinated but contribute to its destabilization.
红系克勒ppel样因子（EKLF/KLF1）是一种红系特异性的C(2)H(2)锌指转录因子，对成人β-珠蛋白基因的正确染色质结构和表达至关重要。在此，我们确定26S蛋白酶体抑制剂导致EKLF蛋白在小鼠红白血病（MEL）细胞中积累。此外，在这些抑制剂存在的情况下，MEL细胞（<3小时）和胎肝细胞（6至9小时）中的EKLF半衰期均得到稳定。EKLF在体内被泛素化，但其修饰并不依赖于特定的内部赖氨酸。最后，EKLF在其N端含有两个PEST序列，这些序列对EKLF被泛素化的能力没有影响，但有助于其不稳定。
