Bettendorff L
Laboratory of General and Comparative Biochemistry, University of Liège, Belgium.
J Chromatogr. 1991 May 31;566(2):397-408. doi: 10.1016/0378-4347(91)80256-c.
Thiamine triphosphate can be found in most tissues at very low levels, but its role is unknown. Organs and muscles that generate electrical impulses are particularly rich in this compound. This paper describes a thiamine triphosphatase from the electrical organ of Electrophorus electricus. The activity of this enzyme, as measured by a high-performance liquid chromatographic method, is closely anion-regulated. Furthermore, thiamine triphosphate increases chloride uptake in membrane vesicles prepared from rat brain. Our results suggest that this compound could play an important role in the regulation of chloride permeability.
三磷酸硫胺素在大多数组织中的含量极低，但其作用尚不清楚。产生电冲动的器官和肌肉中这种化合物的含量尤为丰富。本文描述了一种来自电鳗电器官的三磷酸硫胺素酶。通过高效液相色谱法测定，该酶的活性受到阴离子的密切调节。此外，三磷酸硫胺素可增加从大鼠脑制备的膜囊泡对氯化物的摄取。我们的结果表明，这种化合物可能在氯化物通透性的调节中起重要作用。
