Clair R W, Toma J J, Lofland H B
Atherosclerosis. 1975 Mar-Apr;21(2):155-65. doi: 10.1016/0021-9150(75)90077-5.
Proline hydroxylase activity and collagen content were determined in atherosclerotic plaque, fatty streak, and normal tissue from aortas of White Carneau pigeons with naturally-occurring of cholesterol-aggravated atherosclerosis. Little increase in collagen content or proline hydroxylase activity occurred in fatty streaks or plaques from birds with cholesterol-aggravated atherosclerosis. This is consistent with the morphologic observation of the presence of little or no "fibromuscular cap" in these cholesterol-aggravated lesions. Both normal and plaque tissue from arotas of birds with naturally-occurring atherosclerosis contained more collagen than did similar tissues from control birds or birds with cholesterol-aggravated lesions. The largest proportion of this increase in collagen content probably represented an age effect since it occurred in normal as well as atherosclerotic tissue. Plaques from aortas of birds with naturally-occurring atherosclerosis did contain, however, significantly more collagen than normal tissue from the same aortas. This is consistent with the presence of a prominent "fibromuscular cap" in these naturally-occurring lesions. Proline hydroxylase activity was less in these lesions than in normal tissue from the same aortas. Consequently increased proline hydroxylase activity and collagen content are not greatly altered in association with development of cholesterol-aggravated atherosclerotic lesions in pigeons. On the other hand, well-developed naturally-occurring lesions contained increased concentrations of collagen but showed no increase proline hydroxylase activity. This is not to say though, that active collagen synthesis and presukably increased proline hydroxylase activity did not take place at some point in the development of these naturally-occurring lesions.
在患有自然发生的胆固醇加重型动脉粥样硬化的白卡诺鸽主动脉的动脉粥样硬化斑块、脂肪条纹和正常组织中，测定了脯氨酸羟化酶活性和胶原蛋白含量。患有胆固醇加重型动脉粥样硬化的鸟类的脂肪条纹或斑块中，胶原蛋白含量或脯氨酸羟化酶活性几乎没有增加。这与在这些胆固醇加重型病变中几乎没有或不存在“纤维肌帽”的形态学观察结果一致。患有自然发生的动脉粥样硬化的鸟类主动脉的正常组织和斑块组织比对照鸟类或患有胆固醇加重型病变的鸟类的类似组织含有更多的胶原蛋白。胶原蛋白含量增加的最大比例可能代表年龄效应，因为它在正常组织和动脉粥样硬化组织中均有发生。然而，患有自然发生的动脉粥样硬化的鸟类主动脉斑块确实比同一主动脉的正常组织含有明显更多的胶原蛋白。这与这些自然发生的病变中存在明显的“纤维肌帽”一致。这些病变中的脯氨酸羟化酶活性低于同一主动脉的正常组织。因此，脯氨酸羟化酶活性和胶原蛋白含量的增加与鸽子胆固醇加重型动脉粥样硬化病变的发展没有太大关联。另一方面，发育良好的自然发生的病变中胶原蛋白浓度增加，但脯氨酸羟化酶活性没有增加。不过，这并不是说在这些自然发生的病变发展的某个阶段没有发生活跃的胶原蛋白合成以及推测的脯氨酸羟化酶活性增加。
