Ono E, Tomioka Y, Watanabe Y, Amagai K, Taharaguchi S, Glenisson J, Cherel P
The Avian Zoonoses Research Centre, Faculty of Agriculture, Tottori University, Tottori, Japan.
Arch Virol. 2006 Sep;151(9):1827-39. doi: 10.1007/s00705-006-0747-6. Epub 2006 Apr 3.
Nectin-1 is an alphaherpesvirus receptor that binds to virion glycoprotein D (gD). Porcine nectin-1 mediates entry of pseudorabies virus (PRV), herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), and bovine herpesvirus type 1 (BHV-1). The gD-binding domain of nectin-1 is the first or N-terminal immunoglobulin (Ig)-like domain of the entire ectodomain. Here, we generated three transgenic mouse lines expressing a fusion protein consisting of the first Ig-like domain of porcine nectin-1 and the Fc portion of porcine IgG1 to assess the antiviral potential of the first Ig-like domain of nectin-1 in vivo. All of the transgenic mouse lines showed significant resistance to PRV infection via intraperitoneal inoculation (survival rates of 67% to 100%). In the intranasal challenge, a lower but still significant protection was observed; 21% to 55% of the animals from the three transgenic mouse lines survived. The present results demonstrate that a soluble form of the first domain of porcine nectin-1 is able to exert a significant antiviral effect against pseudorabies virus infection.
Nectin-1是一种与病毒粒子糖蛋白D（gD）结合的α疱疹病毒受体。猪Nectin-1介导伪狂犬病病毒（PRV）、1型和2型单纯疱疹病毒（HSV-1和HSV-2）以及1型牛疱疹病毒（BHV-1）的进入。Nectin-1的gD结合结构域是整个胞外结构域的第一个或N端免疫球蛋白（Ig）样结构域。在此，我们构建了三个表达由猪Nectin-1的第一个Ig样结构域和猪IgG1的Fc部分组成的融合蛋白的转基因小鼠品系，以评估Nectin-1的第一个Ig样结构域在体内的抗病毒潜力。所有转基因小鼠品系经腹腔接种后对PRV感染均表现出显著抗性（存活率为67%至100%）。在鼻内攻毒实验中观察到较低但仍显著的保护作用；三个转基因小鼠品系中有21%至55%的动物存活。目前的结果表明，猪Nectin-1第一个结构域的可溶性形式能够对伪狂犬病病毒感染发挥显著的抗病毒作用。
