Wirth J J, Fluck M M
Department of Microbiology, Michigan State University, East Lansing 48824-1101.
J Virol. 1991 Dec;65(12):6985-8. doi: 10.1128/JVI.65.12.6985-6988.1991.
The uniformly lethal development of mammary tumors in polyomavirus-infected adult female nude mice was prevented by adoptive cell transfer of polyomavirus-immune splenocytes or peritoneal cells. Transferred immune cells also lowered the growth rate of emerging tumors. The induction of other relatively less frequent tumors of the skin and bone was decreased as well. Using in situ hybridization of whole-body sections as well as hybridization of nucleic acids from the mammary glands, we show for the first time that transferred immune cells, but not normal cells, virtually eliminated virus signal in the whole mouse and in the mammary glands. Since infected and tumorous mammary glands produce very little infectious virus, it appears that a major mechanism mediating the prevention of polyomavirus oncogenesis involves the immunological elimination of nonproductively and persistently infected cells.
在多瘤病毒感染的成年雌性裸鼠中，通过多瘤病毒免疫脾细胞或腹膜细胞的过继性细胞转移可预防乳腺肿瘤的一致致死性发展。转移的免疫细胞还降低了新出现肿瘤的生长速度。皮肤和骨骼中其他相对较少见肿瘤的诱导也减少了。通过对全身切片进行原位杂交以及对乳腺核酸进行杂交，我们首次表明，转移的免疫细胞而非正常细胞几乎消除了整个小鼠和乳腺中的病毒信号。由于受感染和肿瘤性乳腺产生的感染性病毒极少，似乎介导预防多瘤病毒致癌作用的主要机制涉及对非生产性和持续性感染细胞的免疫清除。
