Cholewa Justyna, Feeney Graham P, O'Reilly Michael, Stürzenbaum Stephen R, Morgan A John, Płytycz Barbara
Department of Evolutionary Immunobiology, Institute of Zoology, Jagiellonian University, Kraków, Poland.
Folia Histochem Cytobiol. 2006;44(1):65-71.
Immunocompetent cells of earthworms, coelomocytes, comprise adherent amoebocytes and granular eleocytes (chloragocytes). Both cell populations can be expelled via dorsal pores of adult earthworms by exposure to an electric current (4.5 V) for 1 min. Analysis by phase contrast/fluorescence microscopy and flow cytometry demonstrated that eleocyte population of several species exhibits a strong autofluorescence. A high percentage (11-35%) of autofluorescent eleocytes was recorded in Allolobophora chlorotica, Dendrodrilus rubidus, Eisenia fetida, and Octolasion sp. (O. cyaneum, O. tyrtaeum tyrtaeum and O. tyrtaeum lacteum). In contrast, autofluorescent coelomocytes were exceptionally scarce (less than 1%) in representative Aporrectodea sp. (A. caliginosa and A. longa) and Lumbricus sp. (L. castaneus, L. festivus, L. rubellus, L. terrestris). Thus, this paper for the first time describes profound intrinsic fluorescence of eleocytes in some--but not all--earthworm species. The function (if any) and inter-species differences of the autofluorescent coelomocytes still remain elusive.
蚯蚓具有免疫活性的细胞，即体腔细胞，包括贴壁变形细胞和颗粒状黄细胞（绿细胞）。通过施加1分钟的电流（4.5伏），这两种细胞群体都可以通过成年蚯蚓的背孔排出。相差/荧光显微镜分析和流式细胞术分析表明，几种蚯蚓的黄细胞群体呈现出强烈的自发荧光。在绿正蚓、红枝蚓、赤子爱胜蚓和八毛蚓属（蓝八毛蚓、硬皮八毛蚓和乳白八毛蚓）中，记录到高比例（11%-35%）的自发荧光黄细胞。相比之下，在代表性的盲蚓属（黑暗盲蚓和长盲蚓）和正蚓属（栗色正蚓、欢快正蚓、红色正蚓、陆正蚓）中，自发荧光体腔细胞极为稀少（不到1%）。因此，本文首次描述了部分（但并非全部）蚯蚓物种黄细胞存在强烈的固有荧光。自发荧光体腔细胞的功能（若有）以及种间差异仍然未知。
