Wang Ya-Dan, Hu Yu, Sun Chun-Yan
Institute of Hematology, Union Hospital Affiliated to Tongji Medcal College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Feb;14(1):70-4.
In order to explore the probability of curcumin treating multiple myeloma (MM) via the inhibition of angiogenesis, the expressions of brain derived neurotrophic factor (BDNF) and its specific receptor in human MM cells and endothelial cells were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The angiogenic activity was evaluated by endothelial cell migration assay and tubule formation assay in vitro. The results showed that exogenous BDNF significantly induced endothelial cell tubule formation and endothelial cell migration, these two effects were inhibited by curcumin. Furthermore, BDNF was detected in the MM cell and TrkB was detected in the endothelial cell and curcumin depressed the mRNA expression of BDNF and TrkB in the dose- and time-dependent manners. It is concluded that BDNF is a novel angiogenesis protein. Curcumin interrupts the interaction between multiple myeloma cells and endothelial cells by reducing TrkB expression in endothelial cells and inhibiting BDNF production in multiple myeloma cells, eventually, resulting in inhibition of angiogenesis. This is probably one part of the mechanism of the curcumin treating MM via the inhibition of angiogenesis.
为了探讨姜黄素通过抑制血管生成治疗多发性骨髓瘤（MM）的可能性，采用逆转录聚合酶链反应（RT-PCR）检测人脑源性神经营养因子（BDNF）及其特异性受体在人MM细胞和内皮细胞中的表达。通过体外内皮细胞迁移试验和小管形成试验评估血管生成活性。结果表明，外源性BDNF显著诱导内皮细胞小管形成和内皮细胞迁移，这两种作用均被姜黄素抑制。此外，在MM细胞中检测到BDNF，在内皮细胞中检测到TrkB，且姜黄素以剂量和时间依赖性方式抑制BDNF和TrkB的mRNA表达。结论：BDNF是一种新型血管生成蛋白。姜黄素通过降低内皮细胞中TrkB的表达并抑制MM细胞中BDNF的产生，中断了MM细胞与内皮细胞之间的相互作用，最终导致血管生成受到抑制。这可能是姜黄素通过抑制血管生成治疗MM机制的一部分。
