Tang Yu-Hong, Fei Xiao-Ming, Shen Wen-Yi, Miao Kou-Rong, Cui Yu-Gui, Wang Cheng-Ya
Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Feb;14(1):89-93.
HOXB4, a member of homeobox gene family, is closely related to the self-renewing and proliferative ability of primitive hematopoietic stem/progenitor cells (PHSC/PHPC). This study was aimed to investigate the self-renewing level of cord blood progenitor cells (CBPC) expanded in vitro. The HOXB4 expression at mRNA level was assayed by using real time RT-PCR. The results indicated that as culture prolonged, the total cells, CD34(+) cells greatly increased, however the HOXB4 expression gradually declined, even down to undetectable level similar to that of mature lymphocytes. Meanwhile, it was shown that CD34(+) cells co-cultured with bone marrow mesenchymal stem cells (BM-MSC) could abate the decline of HOXB4 expression. It is concluded that the self-renewing potential of CD34(+) cells gradually decreased during expansion in vitro, co-culture with BM-MSC was helpful to CD34(+) cell expansion and slowed the loss trend of its self-renewal.
HOXB4是同源框基因家族的成员之一，与原始造血干/祖细胞（PHSC/PHPC）的自我更新和增殖能力密切相关。本研究旨在探讨体外扩增的脐血祖细胞（CBPC）的自我更新水平。采用实时RT-PCR检测HOXB4在mRNA水平的表达。结果表明，随着培养时间的延长，总细胞数、CD34(+)细胞数大幅增加，但HOXB4表达逐渐下降，甚至降至与成熟淋巴细胞相似的不可检测水平。同时发现，与骨髓间充质干细胞（BM-MSC）共培养的CD34(+)细胞可减轻HOXB4表达的下降。结论是，CD34(+)细胞在体外扩增过程中自我更新潜能逐渐降低，与BM-MSC共培养有助于CD34(+)细胞的扩增，并减缓其自我更新的丧失趋势。
