Liu Qin-Guo, Yang Dong-Lin, Huang Yong, Jiang Er-Lie, Zhou Shi-Yong, He Yi, Wang Zhi-Dong, Wang Mei, Zhou Zheng, Zai Weng-Jing, Feng Si-Zhou, Han Min-Ze
Hematologic Hospital, Institute of Hematology, China Union Medical University, Chinese Academy of Medical Sciences, Tianjing 300020, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Feb;14(1):107-11.
To investigate the influence of G-CSF mobilization on functions of donor T lymphocyte subpopulation and acute graft-versus-host disease, peripheral blood samples of 20 healthy donors were collected before and after G-CSF mobilization. The whole blood was diluted with IMDM in ratio of 1:1 and then incubated with PMA + ionomycin + monensin at 37 degrees C, 5% CO2 for 4 hours. After being mobilized and stained, the IL-4, IFN-gamma and IL-2 positive cells were counted with three-color flow cytometry. The results showed that before G-CSF mobilization, the percentages of donor's CD3(+)IFN-gamma(+), CD4(+)IFN-gamma(+), CD8(+)IFN-gamma(+) T cells were 3.2% (0% - 45.9%), 1.3% (0% - 23.8%) and 1.5% (0% - 22.2%) respectively. The percentage of above mentioned cells in donor increased to 19.2% (0% - 53.9%), 9.5% (0% - 49.5%), 7.5% (0% - 38.1%) respectively after G-CSF mobilization. The IL-2 positive CD3(+), CD4(+) and CD8(+) T cell percentage in pre-G-CSF mobilized donors was 1.5% (0% - 31%), 0.8% (0% - 30.0%) and 0% (0% - 5.3%) respectively and subsequently increased to 25.7% (0% - 51%), 19.8% (0% - 39.7%), 4.6% (0% - 20.9%) respectively after G-CSF mobilization. The IL-4 positive T subpopulation did not increased significantly after G-CSF mobilization. In the early stage after peripheral blood stem cell transplantation, donor's Tc1 percentage in aGVHD group was significantly higher than that in non-aGVHD group. The morbidity of severe aGVHD in high Tc2 percentage group was significantly lower than that in low Tc2 percentage group. It is concluded that the donor's type I T cells increase after G-CSF mobilization, the Tc1 percentage of G-CSF mobilized donor is correlated with the occurrence of aGVHD in the early stage after HSCT, the percentage of Tc2 in donor is negatively correlated with aGVHD morbidity in recipients.
为探讨粒细胞集落刺激因子（G-CSF）动员对供体T淋巴细胞亚群功能及急性移植物抗宿主病的影响，采集20例健康供体在G-CSF动员前后的外周血样本。将全血按1:1比例用IMDM稀释，然后在37℃、5%二氧化碳条件下与佛波酯（PMA）+离子霉素+莫能菌素孵育4小时。动员并染色后，采用三色流式细胞术对白细胞介素-4（IL-4）、干扰素-γ（IFN-γ）和白细胞介素-2（IL-2）阳性细胞进行计数。结果显示，在G-CSF动员前，供体的CD3(+)IFN-γ(+)、CD4(+)IFN-γ(+)、CD8(+)IFN-γ(+) T细胞百分比分别为3.2%（0% - 45.9%）、1.3%（0% - 23.8%）和1.5%（0% - 22.2%）。G-CSF动员后，上述细胞在供体中的百分比分别增至19.2%（0% - 53.9%）、9.5%（0% - 49.5%）、7.5%（0% - 38.1%）。在G-CSF动员前的供体中，IL-2阳性的CD3(+)、CD4(+)和CD8(+) T细胞百分比分别为1.5%（0% - 31%）、0.8%（0% - 30.0%）和0%（0% - 5.3%），G-CSF动员后分别增至25.7%（0% - 51%）、19.8%（0% - 39.7%）、4.6%（0% - 20.9%）。G-CSF动员后IL-4阳性T亚群无明显增加。在外周血干细胞移植后的早期，急性移植物抗宿主病（aGVHD）组供体的Tc1百分比显著高于非aGVHD组。高Tc2百分比组严重aGVHD的发病率显著低于低Tc2百分比组。结论：G-CSF动员后供体I型T细胞增加，G-CSF动员供体的Tc1百分比与造血干细胞移植（HSCT）后早期aGVHD的发生相关，供体Tc2百分比与受体aGVHD发病率呈负相关。
