Needleman S B, Goodin K, Severino W
Navy Drug Screening Laboratory, Great Lakes, Illinois 60088.
J Anal Toxicol. 1991 Jul-Aug;15(4):179-81. doi: 10.1093/jat/15.4.179.
Because DuPont Instruments has discontinued commercial availability of the PREP automated sample processor, an alternate method for the general extraction of THC-COOH and benzoylecgonine from a urine matrix in the military drug screening laboratories' standard procedure is needed. The liquid-liquid extraction method presented for isolation of THC-COOH uses extraction with isobutanol-hexane followed by back extraction into an aqueous alkaline medium and results in a percent recovery of 104.24 +/- 9.71%. The isolation of benzoylecgonine involves extraction into ethanol-methylene chloride followed by evaporation and derivatization with 1-iodopropane; recovery is 102.43 +/- 3.13%.
由于杜邦仪器公司已停止提供PREP自动样品处理器，因此需要一种替代方法，以便按照军事药物筛查实验室的标准程序，从尿液基质中常规提取四氢大麻酚-羧酸（THC-COOH）和苯甲酰爱康宁。所提出的用于分离THC-COOH的液液萃取方法是先用异丁醇-己烷萃取，然后反萃取到碱性水介质中，回收率为104.24±9.71%。苯甲酰爱康宁的分离包括萃取到乙醇-二氯甲烷中，然后蒸发并用1-碘丙烷衍生化；回收率为102.43±3.13%。
