Cone R I, Lameh J, Sadée W
School of Pharmacy, University of California, San Francisco 94143.
Life Sci. 1991;49(19):PL147-52. doi: 10.1016/0024-3205(91)90396-s.
We have measured mu and delta opioid receptor sites on intact SK-N-SH and NG108-15 neuroblastoma cells, respectively, in culture. Use of 125I-beta-endorphin (beta E) as a tracer, together with beta E(6-31) to block high-affinity non-opioid binding in both cell lines, permitted the measurement of cell surface mu and delta opioid receptor sites. Labeling was at delta sites in NG108-15 cells and predominantly at mu sites in SK-N-SH cells. Pretreatment with the mu and delta agonist, DADLE, caused a rapid loss of cell surface delta receptor sites in NG108-15 cells, but failed to reduce significantly mu receptor density in SK-N-SH cells.
我们分别测量了培养中的完整SK-N-SH和NG108-15神经母细胞瘤细胞上的μ和δ阿片受体位点。使用¹²⁵I-β-内啡肽（βE）作为示踪剂，并结合βE（6-31）来阻断两种细胞系中的高亲和力非阿片类结合，从而能够测量细胞表面的μ和δ阿片受体位点。在NG108-15细胞中标记位于δ位点，而在SK-N-SH细胞中主要位于μ位点。用μ和δ激动剂DADLE预处理导致NG108-15细胞中细胞表面δ受体位点迅速丧失，但未能显著降低SK-N-SH细胞中的μ受体密度。
