铷（86Rb+）流入对照大鼠和链脲佐菌素诱导的糖尿病大鼠的背根神经节和坐骨神经内膜：与酶促钠钾ATP酶活性的比较。

Rubidium (86Rb+) influx into dorsal root ganglia and sciatic nerve endoneurium of control and streptozotocin-diabetic rats: comparison with enzymatic Na,K-ATPase activity.

作者信息

Llewelyn J G, Patel N J, Wright D W, Thomas P K

机构信息

Department of Neurological Science, Royal Free Hospital School of Medicine, London, England.

出版信息

Metabolism. 1991 Oct;40(10):1079-83. doi: 10.1016/0026-0495(91)90133-h.

DOI:10.1016/0026-0495(91)90133-h

PMID:1658545

Abstract

Streptozotocin (STZ)-induced diabetes in the rat causes a significant reduction in ouabain-sensitive Na,K-ATPase pumping activity measured by 86Rb+ influx, in sciatic endoneurium (by 54%) and dorsal root ganglia (by 22%). For endoneurium, the change is similar to that of ouabain-sensitive enzymatic Na,K-ATPase activity (42%), but in dorsal root ganglia, the decrease in enzymatic Na,K-ATPase activity was much greater. 86Rb+ efflux from dorsal root ganglia showed no difference between diabetic and control animals, confirming that the abnormal 86Rb+ influx reflects Na,K-ATPase function and not abnormal membrane permeability. The significance of these findings to pathogenetic mechanisms in diabetic neuropathy is discussed.

摘要

链脲佐菌素（STZ）诱导的大鼠糖尿病会导致坐骨神经内膜（降低54%）和背根神经节（降低22%）中通过86Rb+内流测量的哇巴因敏感的钠钾ATP酶泵活性显著降低。对于神经内膜，这种变化与哇巴因敏感的酶促钠钾ATP酶活性的变化（42%）相似，但在背根神经节中，酶促钠钾ATP酶活性的降低要大得多。糖尿病动物和对照动物的背根神经节86Rb+外流没有差异，这证实了异常的86Rb+内流反映的是钠钾ATP酶功能，而非异常的膜通透性。本文讨论了这些发现对糖尿病神经病变发病机制的意义。