Kim Beum Jun, Gibson Donna M, Shuler Michael L
School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York 14853, USA.
Biotechnol Bioeng. 2006 Sep 5;95(1):8-14. doi: 10.1002/bit.20934.
Phytosulfokine-alpha (PSK-alpha) is a small plant peptide (5 amino acids) that displays characteristics typically associated with animal peptide hormones. PSK-alpha was originally isolated based on its mitogenic activity with plant cultures; it has been reported to increase production of tropane alkaloids from Atropa belladonna, although its general influence on secondary metabolite production is unknown. The studies reported in this article were initiated to evaluate the effects of PSK-alpha supplementation on production of Taxol (paclitaxel) from plant cell cultures of Taxus sp. particularly when methyl jasmonate (MeJA) is added as an elicitor of secondary metabolism. The response to PSK-alpha supplementation was cell line dependent. Taxus cuspidata P93AF showed no statistically significant response to PSK-alpha supplementation while Taxus canadensis C93AD and T. cuspidata PO93X displayed a concentration-dependent response (up to 100 nM PSK-alpha added in first 24 h of culture) with a decrease in initial growth rate, an increase in cell density (dry weight/fresh weight), and increased Taxol production. More remarkably with T. canadensis (C93AD), a very strong synergistic response of PSK-alpha (100 nM) and methyl jasmonate (MeJA, 100 microM) elicitation was observed, resulting in Taxol level of 35.3 +/- 2.1 mg/L or 1.83 +/- 0.02 mg Taxol/g dry cell weight achieved at day 21, a level of approximately 10-fold higher than for either treatment by itself. Although the level of Taxol production achieved is not remarkable, this synergistic treatment was able to partially revive taxane production in cultures that have lost productivity due to extended time (over 10 years) in continuous subculture.
植物磺肽素-α（PSK-α）是一种小的植物肽（5个氨基酸），具有一些通常与动物肽激素相关的特性。PSK-α最初是根据其对植物培养物的促有丝分裂活性而分离得到的；据报道，它能增加颠茄中托烷生物碱的产量，但其对次生代谢产物产生的总体影响尚不清楚。本文报道的研究旨在评估添加PSK-α对红豆杉属植物细胞培养物中紫杉醇（paclitaxel）产量的影响，特别是在添加茉莉酸甲酯（MeJA）作为次生代谢诱导剂的情况下。对PSK-α添加的反应因细胞系而异。东北红豆杉P93AF对PSK-α添加无统计学显著反应，而加拿大红豆杉C93AD和东北红豆杉PO93X则表现出浓度依赖性反应（在培养的前24小时添加高达100 nM的PSK-α），初始生长速率降低，细胞密度（干重/鲜重）增加，紫杉醇产量增加。更值得注意的是，对于加拿大红豆杉（C93AD），观察到PSK-α（100 nM）和茉莉酸甲酯（MeJA，100 microM）诱导有非常强的协同反应，在第21天达到紫杉醇水平为35.3±2.1 mg/L或1.83±0.02 mg紫杉醇/克干细胞重量，这一水平比单独任何一种处理高出约10倍。尽管所达到的紫杉醇产量水平并不显著，但这种协同处理能够部分恢复因连续传代培养时间延长（超过10年）而失去生产力的培养物中的紫杉烷产量。
