Weber G, Shinitzky M
DEPARTMENT OF CHEMISTRY AND CHEMICAL ENGINEERING, UNIVERSITY OF ILLINOIS, URBANA.
Proc Natl Acad Sci U S A. 1970 Apr;65(4):823-30. doi: 10.1073/pnas.65.4.823.
Electronic energy transfer among identical molecules has been followed by the depolarization of the fluorescence in concentrated solutions as well as in dimers, polymers, and micelle systems. In the many aromatic fluorophores examined, unlike a few nonaromatic ones, transfer is much decreased or altogether undetectable on excitation at the red edge of the absorption spectrum. The phenomenon is not due to the transfer taking place during a small fraction of the total fluorescence lifetime, nor is it explainable by a decrease in overlap of absorption and emission upon edge excitation.
在浓溶液以及二聚体、聚合物和胶束体系中,相同分子间的电子能量转移可通过荧光去极化来跟踪。在所研究的众多芳香族荧光团中,与少数非芳香族荧光团不同,在吸收光谱的红边激发时,转移会大大减少或完全无法检测到。这种现象并非由于转移发生在总荧光寿命的一小部分时间内,也不能用边缘激发时吸收与发射重叠的减少来解释。