Meares C F, Sundberg M W, Baldeschwieler J D
Department of Chemistry, Stanford University, Stanford, California 94305.
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3718-22. doi: 10.1073/pnas.69.12.3718.
The angular correlation of the 173-247 keV gamma-ray cascade after the electron-capture decay of (111)In is strongly perturbed when the 1-p-nitrophenyl-ethylenediaminetetraacetate chelate of (111)In(8+) is added to a solution containing rabbit antibody to dinitrophenyl groups. The radioactive chelate can be displaced by the addition of dinitrophenyllysine or unlabeled chelate. The average association constant between the antibody and the labeled chelate has been estimated from perturbed angular correlation measurements; this value is compared to the results of equilibrium dialysis. These experiments provide good evidence that information concerning macromolecular behavior can be obtained from perturbed angular correlation experiments that use chemically specific labels.
当将铟 - 111的1 - 对硝基苯基 - 乙二胺四乙酸螯合物(铟 - 111(8 +))添加到含有二硝基苯基基团兔抗体的溶液中时,铟 - 111电子俘获衰变后173 - 247千电子伏特γ射线级联的角关联会受到强烈干扰。通过添加二硝基苯基赖氨酸或未标记的螯合物,放射性螯合物可以被取代。已从干扰角关联测量中估计出抗体与标记螯合物之间的平均缔合常数;将该值与平衡透析结果进行比较。这些实验提供了充分的证据,表明可以从使用化学特异性标记的干扰角关联实验中获得有关大分子行为的信息。