Kruse C A, Tin G W, Baldeschwieler J D
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1212-6. doi: 10.1073/pnas.80.5.1212.
The structural integrity of erythrocyte ghosts made by the preswell and slow-dialysis techniques has been studied in vitro by use of gamma-ray perturbed angular correlation (PAC) techniques and also by standard in vitro leakage methods employing sequestered labeled markers. Complexes of 111In3+ and nitrilotriacetate were encapsulated in ghosts made from human, rabbit, rat, and mouse erythrocytes, and their leakage was monitored by both methods. In addition, 125I-labeled bovine serum albumin was encapsulated, and ghost integrity was monitored by conventional leakage measurements. With the PAC technique the percentage of material released from human ghosts was determined quantitatively, and the results were equivalent to those obtained by the conventional method. In addition, at various times after intravenous injection, tissue distribution of the ghosts in the mouse was studied. The percent injected dose per gram of tissue of the labeled surface proteins of erythrocyte ghosts in circulation approximated that of the entrapped labeled albumin. This suggests that the ghost membrane and contents are strongly associated in vivo. Large 125I-labeled bovine serum albumin molecules and small 111In3+-nitrilotriacetate complexes were delivered in high quantitites to the lung initially, and to the liver and spleen. Because erythrocyte ghosts have the ability to entrap a wide range of substances and deliver them to specific organs, ghosts may be preferable to other drug carriers or drug therapy for treatment of certain disorders.
利用γ射线扰动角关联（PAC）技术以及采用隔离标记物的标准体外渗漏方法，在体外研究了通过预膨胀和缓慢透析技术制备的红细胞影的结构完整性。将111In3 +与次氮基三乙酸的络合物包裹在由人、兔、大鼠和小鼠红细胞制成的红细胞影中，并通过这两种方法监测其渗漏情况。此外，还包裹了125I标记的牛血清白蛋白，并通过传统的渗漏测量来监测红细胞影的完整性。使用PAC技术定量测定了从人红细胞影中释放的物质百分比，结果与传统方法获得的结果相当。此外，在静脉注射后的不同时间，研究了小鼠体内红细胞影的组织分布。循环中红细胞影标记表面蛋白每克组织的注射剂量百分比与包裹的标记白蛋白相近。这表明红细胞影膜和内容物在体内紧密相关。大的125I标记牛血清白蛋白分子和小的111In3 + -次氮基三乙酸络合物最初大量输送到肺部，然后输送到肝脏和脾脏。由于红细胞影有能力包裹多种物质并将它们输送到特定器官，因此在治疗某些疾病方面，红细胞影可能比其他药物载体或药物疗法更具优势。
