Strobel G A
Department of Plant Pathology, Montana State University, Bozeman, Mont. 59715.
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1693-6. doi: 10.1073/pnas.70.6.1693.
Helminthosporoside is the host-specific toxin produced by Helminthosporium sacchari, the organism causing eyespot disease on sugarcane. Clones of sugarcane susceptible to the toxin posses a membrane protein that binds the toxin. Membranes of resistant clones do not bind the toxin. In this study, a binding protein from a susceptible clone was compared with its counterpart from a resistant clone. The protein from the resistant clone did not bind the toxin unless it was first treated with mild detergent. The two proteins are antigenically identical, have the same molecular weight, and each contains four subunits. They differ slightly in their electrophoretic mobility and vary with respect to four different aminoacid residues. The basis of resistance of clone H50-7209 to eyespot disease is directly associated with the structurally altered membrane-binding protein.
蠕孢菌素是由甘蔗眼斑病菌产生的宿主特异性毒素，该病菌会引发甘蔗的眼斑病。对该毒素敏感的甘蔗克隆体拥有一种能结合毒素的膜蛋白。抗性克隆体的膜则不结合毒素。在本研究中，将敏感克隆体的一种结合蛋白与其抗性克隆体的对应蛋白进行了比较。抗性克隆体的蛋白除非先用温和去污剂处理，否则不结合毒素。这两种蛋白在抗原性上相同，分子量相同，且每种都包含四个亚基。它们在电泳迁移率上略有不同，并且在四个不同的氨基酸残基方面存在差异。克隆体H50 - 7209对眼斑病的抗性基础与结构改变的膜结合蛋白直接相关。
