Maitra S K, Schotz M C, Yoshikawa T T, Guze L B
Research Service, Veterans Administration, Wadsworth Hospital Center, Los Angeles, California 90073.
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3993-7. doi: 10.1073/pnas.75.8.3993.
A quantitative technique for determining lipid A content of endotoxin added to serum by combined gas chromatography-mass spectrometry is described. This technique uses detection of the beta-hydroxymyristic acid content of Salmonella minnesota R595 lipopolysaccharide by selected ion monitoring at atomic mass unit of 315.4 The fatty acids produced on hydrolysis of serum containing lipopolysaccharide were extracted and the methyl esters were made. Silica gel chromatography was used to separate methyl esters of hydroxy fatty acids from other fatty acid methyl esters. Trimethylsilyl ether derivatives of the hydroxy fatty acid methyl ester fraction were quantitated by this technique. As little as 200 fmol of beta-hydroxymyristic acid could be detected.
描述了一种通过气相色谱 - 质谱联用技术测定添加到血清中的内毒素脂质A含量的定量技术。该技术通过在原子质量单位315.4处进行选择离子监测来检测明尼苏达沙门氏菌R595脂多糖的β-羟基肉豆蔻酸含量。提取含有脂多糖的血清水解产生的脂肪酸并制备甲酯。使用硅胶色谱法将羟基脂肪酸甲酯与其他脂肪酸甲酯分离。通过该技术对羟基脂肪酸甲酯馏分的三甲基硅醚衍生物进行定量。可检测到低至200飞摩尔的β-羟基肉豆蔻酸。