Oldfield E, Gilmore R, Glaser M, Gutowsky H S, Hshung J C, Kang S Y, King T E, Meadows M, Rice D
School of Chemical Sciences, University of Illinois, Urbana, Illinois 61801.
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4657-60. doi: 10.1073/pnas.75.10.4657.
Deuterium Fourier-transform nuclear magnetic resonance spectra have been obtained of 1-myristoyl 2-(14,14,14-trideutero)myristoyl phosphatidylcholine bilayers at 34.1 MHz by using the quadrupole echo pulse technique. Thereby, we have investigated the effects upon the deuterated dimyristoyl phosphatidylcholine bilayers of the following proteins and polypeptides: gramicidin A, bacteriophage f1 coat protein, beef brain myelin proteolipid apoprotein, cytochrome b(5), and cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1). Above T(c), the transition temperature between the gel and liquid crystal phases, the quadrupole splitting of the deuterium-labeled methyl group is reduced or collapsed in the presence of protein or polypeptide. No evidence has been found for ordered "boundary lipid." Below T(c), the spectra show that the hydrocarbon chains are prevented from crystallizing by the protein (or polypeptide) incorporated in the membrane. Similar disordering effects above T(c) are also seen when an unsaturated lipid, 1-(16,16,16-trideutero)palmitoyl 2-palmitoleyl phosphatidylcholine is complexed with cytochrome oxidase.
利用四极回波脉冲技术,在34.1兆赫兹下获得了1-肉豆蔻酰基-2-(14,14,14-三氘代)肉豆蔻酰基磷脂酰胆碱双层膜的氘傅里叶变换核磁共振谱。借此,我们研究了以下蛋白质和多肽对氘代二肉豆蔻酰基磷脂酰胆碱双层膜的影响:短杆菌肽A、噬菌体f1外壳蛋白、牛脑髓鞘蛋白脂蛋白脱辅基蛋白、细胞色素b(5)和细胞色素c氧化酶(亚铁细胞色素c:氧氧化还原酶,EC 1.9.3.1)。在凝胶相和液晶相之间的转变温度T(c)以上,在存在蛋白质或多肽的情况下,氘标记甲基的四极分裂减小或消失。未发现有序“边界脂质”的证据。在T(c)以下,光谱表明膜中掺入的蛋白质(或多肽)阻止了烃链结晶。当不饱和脂质1-(16,16,16-三氘代)棕榈酰基-2-棕榈油酰基磷脂酰胆碱与细胞色素氧化酶复合时,在T(c)以上也观察到类似的无序化效应。