通过2H核磁共振谱矩分析混合脂质相的组成
Analysis of the composition of mixed lipid phases by the moments of 2H NMR spectra.
作者信息
Jarrell H C, Byrd R A, Smith I C
出版信息
Biophys J. 1981 Jun;34(3):451-63. doi: 10.1016/S0006-3495(81)84862-X.
Abstract
The use of (2)H NMR spectral moments to determine the composition of biphasic lipid mixtures is outlined. The analysis has been applied to phosphatidylethanolamine-cholesterol (1:1), potassium palmitate, 30% (wt/wt) water and phosphatidylcholine-cholesterol (4:1) systems, as well as to membrances of Escherichia coli during phase transitions. The advantages and disadvantages of the use of spectral moments to determine fractions of coexistent phases are discussed.
摘要
概述了利用（2）H核磁共振谱矩来确定双相脂质混合物的组成。该分析已应用于磷脂酰乙醇胺 - 胆固醇（1:1）、棕榈酸钾、30%（重量/重量）水和磷脂酰胆碱 - 胆固醇（4:1）体系，以及大肠杆菌膜在相变过程中的情况。讨论了使用谱矩来确定共存相分数的优缺点。
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本文引用的文献
1
Deuterium nuclear magnetic resonance investigation of the effects of proteins and polypeptides on hydrocarbon chain order in model membrane systems.
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4657-60. doi: 10.1073/pnas.75.10.4657.
2
Orientational order of unsaturated lipids in the membranes of Acholeplasma laidlawii as observed by 2H-NMR.
Biochim Biophys Acta. 1980 Aug 4;600(2):245-62. doi: 10.1016/0005-2736(80)90430-7.
3
Quantitative study of the fluidity of Escherichia coli membranes using deuterium magnetic resonance.
Biochemistry. 1980 Feb 5;19(3):451-7. doi: 10.1021/bi00544a008.
4
The temperature dependence of molecular order and the influence of cholesterol in Acholeplasma laidlawii membranes.
Biochim Biophys Acta. 1980 Apr 24;597(3):477-91. doi: 10.1016/0005-2736(80)90221-7.
5
6
Studies on lecithin-cholesterol-water interactions by differential scanning calorimetry and X-ray diffraction.
Biochim Biophys Acta. 1968 Apr 29;150(3):333-40. doi: 10.1016/0005-2736(68)90132-6.
7
The dynamic structure of fatty acyl chains in a phospholipid bilayer measured by deuterium magnetic resonance.
Biochemistry. 1974 Nov 5;13(23):4839-45. doi: 10.1021/bi00720a024.
8
Bilayers of dipalmitoyl-3-sn-phosphatidylcholine. Conformational differences between the fatty acyl chains.
Biochim Biophys Acta. 1975 Sep 16;406(1):1-5. doi: 10.1016/0005-2736(75)90037-1.
9
Lipid phase transitions in cytoplasmic and outer membranes of Escherichia coli.
Biochim Biophys Acta. 1975 May 6;389(2):358-69. doi: 10.1016/0005-2736(75)90328-4.
10
A deuterium nuclear magnetic resonance study of the condensing effect of cholesterol on egg phosphatidylcholine bilayer membranes. I. Perdeuterated fatty acid probes.
Chem Phys Lipids. 1976 Oct;17(2-3 SPEC NO):251-63. doi: 10.1016/0009-3084(76)90070-0.
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