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通过2H核磁共振谱矩分析混合脂质相的组成

Analysis of the composition of mixed lipid phases by the moments of 2H NMR spectra.

作者信息

Jarrell H C, Byrd R A, Smith I C

出版信息

Biophys J. 1981 Jun;34(3):451-63. doi: 10.1016/S0006-3495(81)84862-X.

DOI:10.1016/S0006-3495(81)84862-X
PMID:7018609
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1327487/
Abstract

The use of (2)H NMR spectral moments to determine the composition of biphasic lipid mixtures is outlined. The analysis has been applied to phosphatidylethanolamine-cholesterol (1:1), potassium palmitate, 30% (wt/wt) water and phosphatidylcholine-cholesterol (4:1) systems, as well as to membrances of Escherichia coli during phase transitions. The advantages and disadvantages of the use of spectral moments to determine fractions of coexistent phases are discussed.

摘要

概述了利用(2)H核磁共振谱矩来确定双相脂质混合物的组成。该分析已应用于磷脂酰乙醇胺 - 胆固醇(1:1)、棕榈酸钾、30%(重量/重量)水和磷脂酰胆碱 - 胆固醇(4:1)体系,以及大肠杆菌膜在相变过程中的情况。讨论了使用谱矩来确定共存相分数的优缺点。

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本文引用的文献

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Deuterium nuclear magnetic resonance investigation of the effects of proteins and polypeptides on hydrocarbon chain order in model membrane systems.氘核磁共振研究蛋白质和多肽对模型膜系统中烃链有序性的影响。
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4657-60. doi: 10.1073/pnas.75.10.4657.
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Orientational order of unsaturated lipids in the membranes of Acholeplasma laidlawii as observed by 2H-NMR.通过2H-NMR观察莱氏无胆甾原体膜中不饱和脂质的取向有序性。
Biochim Biophys Acta. 1980 Aug 4;600(2):245-62. doi: 10.1016/0005-2736(80)90430-7.
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Quantitative study of the fluidity of Escherichia coli membranes using deuterium magnetic resonance.利用氘磁共振对大肠杆菌膜流动性进行定量研究。
Biochemistry. 1980 Feb 5;19(3):451-7. doi: 10.1021/bi00544a008.
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The temperature dependence of molecular order and the influence of cholesterol in Acholeplasma laidlawii membranes.莱氏无胆甾原体膜中分子有序性的温度依赖性及胆固醇的影响。
Biochim Biophys Acta. 1980 Apr 24;597(3):477-91. doi: 10.1016/0005-2736(80)90221-7.
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Protein-lipid interactions in biological and model membrane systems. Deuterium NMR of Acholeplasma laidlawii B, Escherichia coli, and cytochrome oxidase systems containing specifically deuterated lipids.生物膜和模型膜系统中的蛋白质-脂质相互作用。含有特定氘代脂质的莱氏无胆甾原体B、大肠杆菌和细胞色素氧化酶系统的氘核磁共振。
J Biol Chem. 1981 Feb 10;256(3):1155-9.
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Studies on lecithin-cholesterol-water interactions by differential scanning calorimetry and X-ray diffraction.通过差示扫描量热法和X射线衍射对卵磷脂-胆固醇-水相互作用的研究。
Biochim Biophys Acta. 1968 Apr 29;150(3):333-40. doi: 10.1016/0005-2736(68)90132-6.
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The dynamic structure of fatty acyl chains in a phospholipid bilayer measured by deuterium magnetic resonance.通过氘磁共振测量磷脂双分子层中脂肪酰链的动态结构。
Biochemistry. 1974 Nov 5;13(23):4839-45. doi: 10.1021/bi00720a024.
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Bilayers of dipalmitoyl-3-sn-phosphatidylcholine. Conformational differences between the fatty acyl chains.二棕榈酰-3- sn -磷脂酰胆碱双层膜。脂肪酰链之间的构象差异。
Biochim Biophys Acta. 1975 Sep 16;406(1):1-5. doi: 10.1016/0005-2736(75)90037-1.
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Lipid phase transitions in cytoplasmic and outer membranes of Escherichia coli.大肠杆菌细胞质膜和外膜中的脂质相变
Biochim Biophys Acta. 1975 May 6;389(2):358-69. doi: 10.1016/0005-2736(75)90328-4.
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A deuterium nuclear magnetic resonance study of the condensing effect of cholesterol on egg phosphatidylcholine bilayer membranes. I. Perdeuterated fatty acid probes.胆固醇对卵磷脂酰胆碱双层膜凝聚效应的氘核磁共振研究。I. 全氘代脂肪酸探针。
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