Cerdonio M, Congiu-Castellano A, Calabrese L, Morante S, Pispisa B, Vitale S
Facoltà di Scienze, Free University of Trento, POVO, Trento, Italy.
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4916-9. doi: 10.1073/pnas.75.10.4916.
The magnetic susceptibility and the density of human oxy-(HbO(2)) and carbonmonoxyhemoglobin (HbCO) solutions of various concentrations have been measured at room temperature, with pure water used as a calibrant. Solutions of unstripped and stripped HbO(2) at pH 7.2 in unbuffered water solvent were always found to be less diamagnetic than pure water, whereas solutions of HbCO in identical conditions were always found to be more diamagnetic than pure water. After correcting for concentration-dependent density changes and assuming the HbCO samples to be fully diamagnetic, the paramagnetic reduction of the diamagnetic susceptibility of HbO(2) corresponds to a molar susceptibility per heme (chi(M) (heme)) of 2460 +/- 600 x 10(-6) cgs/mol.
在室温下,以纯水作为校准物,测量了不同浓度的人氧合血红蛋白(HbO₂)和碳氧血红蛋白(HbCO)溶液的磁化率和密度。在无缓冲水溶剂中,pH值为7.2的未去除和去除后的HbO₂溶液总是比纯水的抗磁性小,而在相同条件下的HbCO溶液总是比纯水的抗磁性大。在校正了浓度依赖性密度变化并假设HbCO样品完全抗磁性后,HbO₂抗磁磁化率的顺磁降低对应于每个血红素的摩尔磁化率(χM(血红素))为2460±600×10⁻⁶厘米克秒制/摩尔。