Institut für Biologie III, University of Freiburg, Schänzlestrasse 1, D-7800 Freiburg, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7311-4. doi: 10.1073/pnas.77.12.7311.
The active ATP-dependent uptake of Ca(2+) into a plasma membrane-enriched microsomal fraction from plants is stimulated by the addition of plant as well as bovine brain calmodulin. This stimulation occurs only when the microsomes are prepared in the presence of EDTA. Plant calmodulin also activates the bovine brain 3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17). Mitochondrial Ca(2+) accumulation is not affected by calmodulin. The calmodulin-mediated activation of microsomal Ca(2+) uptake is abolished by the antipsychotic drug fluphenazine, whereas the calmodulin-independent Ca(2+) uptake is only slightly decreased.
植物质膜富含微粒体部分的主动、ATP 依赖的 Ca(2+)摄取,可被外加的植物和牛脑钙调蛋白所刺激。这种刺激仅发生于微粒体在 EDTA 存在下被制备时。植物钙调蛋白还可激活牛脑 3',5'-环核苷酸磷酸二酯酶(EC3.1.4.17)。线粒体 Ca(2+)积累不受钙调蛋白影响。抗精神病药物氟奋乃静可消除钙调蛋白介导的微粒体 Ca(2+)摄取的激活,而钙调蛋白非依赖的 Ca(2+)摄取仅稍有减少。