Stosic V, Penel C, Marme D, Greppin H
Laboratoire de Physiologie végétale, Université de Genève, 1211 Genève 4, Switzerland.
Plant Physiol. 1983 Aug;72(4):1136-8. doi: 10.1104/pp.72.4.1136.
A microsomal fraction isolated from green spinach leaves exhibited a Mg(2+) and ATP-dependent (45)Ca(2+) uptake. Addition of 10 micromolar carbonyl cyanide m-chlorophenylhydrazine had no effect. The cationophore A23187 (10 micromolar) induced the release of (45)Ca(2+) accumulated by membrane vesicles. Membranes prepared from lower epidermis showed the highest Ca(2+) accumulation activity. Microsomal fractions from petiole, lamina, and midrib were less active. The stimulation by bovine brain calmodulin was about 30% for the lower epidermis, 23.5% for midrib, and below 20% for petiole and lamina.
从绿色菠菜叶中分离出的微粒体部分表现出对Mg(2+)和ATP依赖的(45)Ca(2+)摄取。添加10微摩尔的羰基氰化物间氯苯腙没有影响。阳离子载体A23187(10微摩尔)诱导了膜囊泡积累的(45)Ca(2+)的释放。由下表皮制备的膜显示出最高的Ca(2+)积累活性。叶柄、叶片和叶脉的微粒体部分活性较低。牛脑钙调蛋白对下表皮的刺激约为30%,对叶脉为23.5%,对叶柄和叶片则低于20%。