Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Proc Natl Acad Sci U S A. 1981 May;78(5):3083-7. doi: 10.1073/pnas.78.5.3083.
Proteins of ribosomes from various stages of development in Dictyostelium discoideum were analyzed by two-dimensional polyacrylamide gel electrophoresis. Significant changes in protein composition were observed; the data demonstrate that cell differentiation in a eukaryotic system is accompanied by ribosome heterogeneity. Both qualitative and quantitative differences were noted for 12 unique ribosomal proteins between the vegetative amoebae and spores (differentiated cells). Two proteins were specific to ribosomes of amoebae, and three were specific to spores. The others were common to both cells but showed characteristic stoichiometric changes. The appearance and quantitative changes of these proteins were associated with specific stages of cell differentiation and were evident only during the aggregation phase; however, further changes continued through construction of fruiting bodies. As functional mRNAs for all 12 proteins were present in both amoebae and spores, both transcriptional and translational mechanisms apparently regulate the synthesis of the various developmentally controlled ribosomal proteins in the two cell types.
用二维聚丙烯酰胺凝胶电泳分析了粘菌（Dictyostelium discoideum）不同发育阶段的核糖体蛋白。观察到蛋白质组成的显著变化；数据表明，真核系统中的细胞分化伴随着核糖体的异质性。在营养变形虫和孢子（分化细胞）之间，12 种独特的核糖体蛋白存在定性和定量差异。两种蛋白质是变形虫核糖体特有的，三种蛋白质是孢子特有的。其他蛋白质在两种细胞中都很常见，但表现出特征性的化学计量变化。这些蛋白质的出现和数量变化与细胞分化的特定阶段有关，仅在聚集阶段明显；然而，进一步的变化仍在继续，直到形成子实体。由于所有 12 种蛋白质的功能性 mRNA 都存在于变形虫和孢子中，转录和翻译机制显然调节两种细胞类型中各种发育控制的核糖体蛋白的合成。
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