植物的 RNA 依赖性 RNA 聚合酶。
RNA-dependent RNA polymerases of plants.
机构信息
Virus Laboratory and Department of Molecular Biology, University of California, Berkeley, California 94720.
出版信息
Proc Natl Acad Sci U S A. 1983 Jan;80(2):422-4. doi: 10.1073/pnas.80.2.422.
Abstract
The existence of RNA-dependent RNA polymerases (EC 2.7.7.48) in plants has been definitely proven by their isolation in pure form from cucumber and tobacco in our laboratory and from cowpea at Wageningen. These enzymes are single-chain proteins of 100-130 kilodaltons. They show clear physical and biochemical differences characteristic for a given plant species, even when their amounts in the plants were greatly increased prior to isolation by infection with the same virus. The role of these enzymes in plant physiology remains unknown.
摘要
在我们实验室中，已经通过从黄瓜和烟草中以及在瓦赫宁根的豇豆中分离出的纯形式，明确证明了植物中存在 RNA 依赖性 RNA 聚合酶（EC 2.7.7.48）。这些酶是 100-130 千道尔顿的单链蛋白质。即使在感染相同病毒之前，通过感染使这些酶在植物中的含量大大增加，它们仍表现出与特定植物物种相关的明显的物理和生化差异。这些酶在植物生理学中的作用尚不清楚。
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