Institut de Microbiologie, Batiment 409, Université Paris XI 91405, Orsay Cedex 05, France.
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3586-90. doi: 10.1073/pnas.82.11.3586.
During synchronous replication induced in a temperature-sensitive initiation mutant of Bacillus subtilis, we previously isolated an RNA covalently linked to DNA. This molecule was synthesized at specific times, correlated with the period of transcription that is required to initiate a new round of replication. In this paper, we show that both RNA and DNA components of the RNA-DNA molecule hybridized with the coding strand for ribosomal RNA. Competition hybridization experiments also demonstrated that ribosomal RNA sequences represent the great majority of the RNA that is linked to DNA. Both RNA and DNA components of the RNA-DNA molecule also hybridized with a region close to the origin of replication, in particular with E19 and E22, two restriction fragments that are replicated early. These fragments in fact form part of the ribosomal operon rrnO. The role of the RNA-linked DNA molecule in initiation in B. subtilis and the possibility that this molecule emanates directly from rrnO rather than from other ribosomal RNA genes are discussed.
在枯草芽孢杆菌温度敏感起始突变体中诱导的同步复制过程中，我们之前分离到一种与 DNA 共价连接的 RNA。这种分子在特定时间合成，与启动新一轮复制所需的转录时期相关。在本文中，我们表明 RNA-DNA 分子的 RNA 和 DNA 成分均与核糖体 RNA 的编码链杂交。竞争杂交实验还表明，核糖体 RNA 序列代表与 DNA 连接的绝大多数 RNA。RNA-DNA 分子的 RNA 和 DNA 成分也与复制起点附近的区域杂交，特别是与 E19 和 E22 两个早期复制的限制片段杂交。这些片段实际上是核糖体操纵子 rrnO 的一部分。本文讨论了 RNA 连接的 DNA 分子在枯草芽孢杆菌起始中的作用，以及该分子是否直接源自 rrnO 而不是其他核糖体 RNA 基因的可能性。
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