Winsten J A, Huang P C
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1387-91. doi: 10.1073/pnas.69.6.1387.
A protein-DNA transcription complex, isolated from logarithmic-phase cells of Bacillus subtilis, is active in the initiation of new rounds of RNA synthesis in vitro. Transcription directed by the endogenous DNA of the complex is sensitive to inhibitors of initiation. In addition, the effect of addition of a competing template indicates that RNA polymerase in the complex is bound to endogenous DNA in a dissociable, preinitiation state. Hybridization-competition analysis of the RNA product obtained from the in vitro reaction indicates that it is greatly enriched for ribosomal RNA sequences. This initiating complex should be useful for the study of regulation of expression of ribosomal RNA genes.
从枯草芽孢杆菌对数期细胞中分离出的一种蛋白质-DNA转录复合物，在体外新一轮RNA合成的起始过程中具有活性。该复合物内源DNA指导的转录对起始抑制剂敏感。此外，添加竞争模板的效果表明，复合物中的RNA聚合酶以可解离的起始前状态与内源DNA结合。对体外反应获得的RNA产物进行杂交竞争分析表明，其核糖体RNA序列显著富集。这种起始复合物对于研究核糖体RNA基因表达的调控应该是有用的。
