Laboratory of Population Genetics, Department of Biology, Kyushu University, Fukuoka 812, Japan.
Proc Natl Acad Sci U S A. 1987 Nov;84(21):7605-8. doi: 10.1073/pnas.84.21.7605.
The P element is a type of transposable element in Drosophila melanogaster. Characteristics of the syndrome of "hybrid dysgenesis" are due to transposition of P elements, and the molecular mechanism for regulation of this transposition has been unknown. In this study a Q strain (which carries only defective P elements in its genome but still is able to repress the transposition of complete P elements although defective in transposase activity) was used to determine the structure of the P element with this repressor (or P cytotype-determining) domain. Examination of the cytotype and structure of the P elements of particular strains with reduced copy number of P elements showed that the P element with a repressor domain was defective, being deleted between bases 1991 and 2448. This region corresponds to most of the third intron [between open reading frame (ORF) 2 and ORF 3] as well as half the ORF 3 of an intact P element. Therefore ORF 3 was deemed to be unnecessary for repressor production.
P 因子是黑腹果蝇（Drosophila melanogaster）中转座子的一种类型。“杂种不育”综合征的特征是由于 P 因子的转座引起的，而调节这种转座的分子机制尚不清楚。在这项研究中，使用 Q 品系（其基因组中仅携带缺陷型 P 因子，但尽管转座酶活性有缺陷，仍能够抑制完整 P 因子的转座）来确定带有这种抑制剂（或 P 细胞型决定）域的 P 因子的结构。对具有降低 P 因子拷贝数的特定菌株的 P 因子的细胞型和结构的检查表明，具有抑制剂域的 P 因子是有缺陷的，在碱基 1991 和 2448 之间缺失。该区域对应于完整 P 因子的第三个内含子[在开放阅读框（ORF）2 和 ORF 3 之间]的大部分以及 ORF 3 的一半。因此，ORF 3 被认为对于抑制剂的产生是不必要的。
Zotero 插件
