Max-Planck-Institut für Züchtungsforschung, Abt. Schell, D-5000 Cologne 30, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1988 Nov;85(22):8578-82. doi: 10.1073/pnas.85.22.8578.
The nodB gene of Rhizobium meliloti encodes a 23.8-kDa protein that is conserved in several Rhizobium species. Monospecific polyclonal antibodies against NodB were used to localize this protein in the cytosol of R. meliloti and Escherichia coli cells containing nodABC genes. In comparison to the NodA and NodC proteins, NodB is synthesized in a disproportionately low amount. The NodA and NodB proteins are involved in generating small, heat-stable compounds that stimulate the mitosis of various plant protoplasts. Our experiments suggest that NodC is not involved in the synthesis of the factors. On the basis of their properties, we speculate that the factors are cytokinin-like substances.
苜蓿中华根瘤菌 nodB 基因编码一个 23.8kDa 的蛋白质，该蛋白在几种根瘤菌中保守。针对 NodB 的单克隆多克隆抗体被用于定位含有 nodABC 基因的苜蓿中华根瘤菌和大肠杆菌细胞中的该蛋白。与 NodA 和 NodC 蛋白相比，NodB 的合成量不成比例地低。NodA 和 NodB 蛋白参与产生小的、热稳定的化合物，刺激各种植物原生质体的有丝分裂。我们的实验表明，NodC 不参与这些因子的合成。基于它们的特性，我们推测这些因子是细胞分裂素样物质。
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