Department of Plant Biology, University of Zürich, 8008 Zürich, Switzerland.
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9529-32. doi: 10.1073/pnas.85.24.9529.
Senescing barley leaves accumulate a series of pink pigments with the chemical properties of catabolites derived from chlorophyll. Levels of the major component of this group of pigments were quantified by HPLC and shown to be maximal in tissues exhibiting maximal rates of chlorophyll degradation. Protoplasts were isolated from senescent leaf tissue and fractionated to yield intact vacuoles and plastids. Although small but significant proportions both of total catabolites and of the dominant component of the series were recovered from the plastid fraction, the vast bulk of these compounds could be assigned to the vacuole. These observations suggest a role for the vacuole in the later stages of chlorophyll breakdown during senescence.
衰老的大麦叶片积累了一系列具有叶绿素降解产物化学性质的粉红色色素。用高效液相色谱法对该组色素的主要成分进行了定量分析,结果表明,在表现出最大叶绿素降解速率的组织中,该成分的含量最高。从衰老的叶片组织中分离原生质体,并进行分级分离以获得完整的液泡和质体。尽管从质体部分回收了总代谢产物和该系列主要成分的小但显著比例,但这些化合物的大部分可归因于液泡。这些观察结果表明液泡在衰老过程中叶绿素分解的后期阶段起作用。