Department of Biochemistry and Biophysics, University of California, Davis, CA 95616.
Proc Natl Acad Sci U S A. 1989 Aug;86(15):5778-80. doi: 10.1073/pnas.86.15.5778.
The phytochrome holoprotein of plants requires a covalently bound linear tetrapyrrole (bilin) prosthetic group for its photoreceptor function. Here we show that synthetic phytochrome apoprotein prepared by transcription and translation of an Avena phytochrome cDNA construct combines in vitro with phycocyanobilin, an analog of the natural chromophore, to produce a photoactive holoprotein. These results indicate that holoprotein assembly is an "autocatalytic" process.
植物的光敏色素全蛋白需要一个共价结合的线性四吡咯（bilin）辅基才能发挥其光受体功能。在这里，我们展示了通过转录和翻译燕麦光敏色素 cDNA 构建体制备的合成光敏色素脱辅基蛋白与藻红胆素（phycocyanobilin）结合，生成光活性全蛋白。这些结果表明全蛋白组装是一个“自催化”过程。
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