血小板反应蛋白1（TSP 1）在子宫平滑肌肿瘤患者中的表达：一项免疫组织化学研究。

Expression of thrombospondin 1 (TSP 1) in patients with uterine smooth muscle tumors: an immunohistochemical study.

作者信息

Bodner-Adler Barbara, Nather Andreas, Bodner Klaus, Czerwenka Klaus, Kimberger Oliver, Leodolter Sepp, Mayerhofer Klaus

机构信息

Department of Gynecology and Obstetrics, University of Vienna Medical School, A-1090 Vienna, Währinger Gürtel 18-20, Austria.

出版信息

Gynecol Oncol. 2006 Oct;103(1):186-9. doi: 10.1016/j.ygyno.2006.02.023. Epub 2006 Apr 3.

DOI:10.1016/j.ygyno.2006.02.023

PMID:16595146

Abstract

OBJECTIVE

Angiogenesis is an essential component for tumor development regulated by both proangiogenic and antiangiogenic factors. Thrombospondin 1 (TSP 1) suppresses angiogenesis by inhibiting endothelial cell proliferation and inducing endothelial cell apoptosis. The aim of this study was to compare the expression of TSP 1 in cases with leiomyoma, uterine smooth muscle tumor of uncertain malignant potential (STUMP) and leiomyosarcoma (LMS). Furthermore, we evaluated the prognostic relevance of TSP 1 in uterine LMS.

METHODS

TSP 1 expression was investigated by immunohistochemistry from paraffin-embedded tissue in 26 patients with leiomyoma, in 24 patients with STUMP and in 21 patients with LMS. Standard immunohistochemical techniques were used to study the expression of TSP 1 in 5-mum-thick tumor sections. TSP 1 expression was correlated with survival using the Kaplan-Meier method and log-rank test for univariate analysis.

RESULTS

TSP 1 was expressed in 77% of leiomyomas, in 13% of STUMP and in 24% of LMS. A statistically significant difference regarding the frequency of TSP 1 expression was observed between leiomyoma and LMS (P < 0.05) as well as between leiomyoma and STUMP (P < 0.05), but not between LMS and STUMP (P > 0.05). Furthermore, a statistically significant correlation between vascular space involvement and TSP 1 expression was observed in patients with uterine LMS, with patients without vascular space involvement having more frequently TSP 1 positive tumors (P = 0.04). No statistically significant correlation between TSP 1 and clinical stage, age and recurrence disease could be detected (P > 0.05).

CONCLUSIONS

We found that TSP 1 was more frequently expressed in leiomyoma compared to STUMP and LMS. Additionally, the statistically significant negative correlation between vascular space involvement and TSP 1 expression in patients with uterine LMS shows that TSP 1 might work as a predictive factor in patients with LMS. Further clinical studies are necessary to prove our results and to clarify the role of TSP 1 in uterine smooth muscle tumors.

摘要

目的

血管生成是肿瘤发展的一个重要组成部分，受促血管生成因子和抗血管生成因子的共同调控。血小板反应蛋白1（TSP 1）通过抑制内皮细胞增殖和诱导内皮细胞凋亡来抑制血管生成。本研究的目的是比较TSP 1在平滑肌瘤、恶性潜能不确定的子宫平滑肌肿瘤（STUMP）和平滑肌肉瘤（LMS）病例中的表达情况。此外，我们评估了TSP 1在子宫LMS中的预后相关性。

方法

采用免疫组织化学方法，对26例平滑肌瘤患者、24例STUMP患者和21例LMS患者石蜡包埋组织中的TSP 1表达进行研究。使用标准免疫组织化学技术研究5微米厚肿瘤切片中TSP 1的表达。采用Kaplan-Meier法和对数秩检验对TSP 1表达与生存情况进行单因素分析。

结果

TSP 1在77%的平滑肌瘤、13%的STUMP和24%的LMS中表达。在平滑肌瘤与LMS之间（P < 0.05）以及平滑肌瘤与STUMP之间（P < 0.05）观察到TSP 1表达频率存在统计学显著差异，但在LMS与STUMP之间未观察到（P > 0.05）。此外，在子宫LMS患者中观察到血管间隙受累与TSP 1表达之间存在统计学显著相关性，无血管间隙受累的患者TSP 1阳性肿瘤更为常见（P = 0.04）。未检测到TSP 1与临床分期、年龄和复发疾病之间存在统计学显著相关性（P > 0.05）。