Maw M K, Fujimoto J, Tamaya T
Department of Obstetrics and Gynecology, Graduate School of Medicine, Gifu University School of Medicine, Gifu City, Japan.
Br J Cancer. 2008 Nov 18;99(10):1557-63. doi: 10.1038/sj.bjc.6604722. Epub 2008 Oct 28.
The ID protein, an inhibitor of basic helix-loop-helix (HLH) transcription factors, has been involved in multiple cellular processes. To investigate the association between tumour advancement and ID expressions of uterine cervical cancers, the levels of ID-1, ID-2 and ID-3 mRNAs were determined by real-time reverse transcription-polymerase chain reaction and the histoscore with the localisation of ID-1 was determined by immunohistochemistry and patient survival in 60 patients. ID-1 histoscores and mRNA levels both significantly (P<0.05) increased in uterine cervical cancers according to clinical stage regardless of histopathological type or lymph node metastasis. Furthermore, the 36-month survival rate of the 30 patients with high ID-1 was poor (60%), whereas that of the other 30 patients with low ID-1 was significantly higher (83%). ID-1 histoscores and mRNA levels significantly (P<0.0001) correlated with microvessel counts in uterine cervical cancers. Tumour cells show mostly diffuse to strong cytoplasmic expression of ID-1 and also very faint expression in endothelial cells. Moreover, ID-1 expression not only correlated with microvessel counts but also correlated significantly with histoscore. Therefore, ID-1 might work on tumour advancement through angiogenic activity and is considered to be a candidate for a prognostic indicator in uterine cervical cancers.
ID蛋白是一种碱性螺旋-环-螺旋（HLH）转录因子的抑制剂，参与了多种细胞过程。为了研究宫颈癌肿瘤进展与ID表达之间的关联，采用实时逆转录-聚合酶链反应测定了60例患者的ID-1、ID-2和ID-3 mRNA水平，并通过免疫组织化学和患者生存率测定了ID-1定位的组织学评分。无论组织病理学类型或淋巴结转移情况如何，宫颈癌中ID-1组织学评分和mRNA水平均根据临床分期显著（P<0.05）升高。此外，30例ID-1高表达患者的36个月生存率较低（60%），而另外30例ID-1低表达患者的36个月生存率则显著较高（83%）。宫颈癌中ID-1组织学评分和mRNA水平与微血管计数显著（P<0.0001）相关。肿瘤细胞大多表现为ID-1的弥漫性至强细胞质表达，内皮细胞中也有非常微弱的表达。此外，ID-1表达不仅与微血管计数相关，还与组织学评分显著相关。因此，ID-1可能通过血管生成活性作用于肿瘤进展，被认为是宫颈癌预后指标的候选者。
